DNA manufacturing, storage, and access system
A system includes a synthesizer unit having a fluid input to receive fluids and a communication input to receive commands to synthesize data-encoded DNA sequences and cleave the DNA. A first flexible chemistry reaction chamber module may be fluidically coupled to the synthesizer unit to receive the data-encoded DNA sequences and amplify the sequences. A deposition unit may be fluidically coupled to the first flexible chemistry reaction chamber module to receive the amplified DNA sequences and encapsulate the amplified DNA sequences into one or more wells in a storage plate for storage and retrieval to and from a plate storage unit. Retrieved DNA may be processed and read by further units.
1. A system comprising:
a synthesis unit chamber comprising at least one wafer mounted within the synthesis unit chamber and having a plurality of physically-distinct substrates on the wafer for solid-phase synthesis of DNA, the synthesis unit chamber having a fluid input to receive fluids and a fluid output to remove fluids;
a Polymerase Chain Reaction (PCR) module fluidically coupled to the fluid output of the synthesis unit chamber, the PCR module comprising a reaction chamber and a fluidic flow path having an inlet and outlet, wherein the reaction chamber is configured to receive DNA from the synthesis unit chamber and PCR reagents and the fluidic flow path is configured to circulate liquid around the reaction chamber to perform PCR amplification of the DNA thereby generating amplified DNA;
a deposition unit fluidically coupled to the PCR module to receive the amplified DNA and deposit the amplified DNA into a receptacle; and
a controller communicatively coupled to one or more actuators that open and close valves connected to the synthesis unit chamber, the PCR module, and the deposition unit and communicatively coupled to one or more electronic and/or mechanical sensors that sense conditions on the wafer, wherein the controller is programmed to regulate flow of fluids between the synthesis unit chamber, the PCR module, and the deposition unit by operation of the valves.
2. The system of claim 1 , further comprising a rehydration unit configured to receive dried DNA from a well in a storage plate, the rehydration unit comprising a fluid input to receive rehydration solution and a chamber to maintain the rehydration solution in contact with the dried DNA thereby generating rehydrated DNA.
3. The system of claim 2 , further comprising a second PCR module fluidically coupled to the rehydration unit and configured receive the rehydrated DNA from the rehydration unit, wherein the second PCR module is configured to perform PCR amplification of the rehydrated DNA thereby generating amplified DNA, the second PCR module comprising a reaction chamber and a fluidic thermal loop, wherein the reaction chamber is configured to receive rehydrated DNA from the rehydration unit and PCR reagents and the fluidic thermal loop is configured to circulate liquid around the reaction chamber to perform PCR amplification of the rehydrated DNA thereby generating amplified DNA.
4. The system of claim 3 , further comprising a sequencing unit fluidically coupled to the second PCR module and configured to receive the amplified DNA from the second PCR module, wherein the sequencing unit comprises at least one wafer having one or more nanopores configured to read a sequence of DNA deposited on the wafer.
5. The system of claim 4 , wherein the controller is communicatively coupled to the sequencing unit and programmed to decode the sequence of DNA from the sequencing unit into binary output.
6. The system of claim 1 , further comprising a storage plate holder with multiple slots, a one of the multiple slots configured to retrievably hold a storage plate.
7. The system of claim 1 , wherein the at least one wafer is round and mounted within the synthesis unit chamber.
8. The system of claim 1 , wherein the PCR module comprises a linear actuator connected to a rod and configured to agitate the reaction chamber by moving the rod connected to the reaction chamber laterally.
9. The system of claim 7 , further comprising a rotary servo drive coupled to the at least one wafer.