DNase variants

1. A DNase variant, which comprises two or more substitutions selected from the group consisting of: T1I, T1L, T1V, S13Y, T22P, S25P, S27L, S39P, S42G, S42A, S42T, S57W, S57Y, S57F, S59V, S59I, S59L, V76L, V76I, Q109R, S116D, S116E, T127V, T127I, T127L, S144P, A147H, S167L, S167I, S167V, G175D and G175E, wherein

the positions correspond to the positions of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1),

the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 or the polypeptide shown in SEQ ID NO: 28 of at least 90%, and

the variant has DNase activity.

2. The DNase variant of claim 1 , wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO: 1 or the polypeptide shown in SEQ ID NO: 28 of at least 95%, and the variant has DNase activity.

3. The DNase variant according to claim 1 wherein the variant comprises one or more of the substitution sets selected from the group consisting of: T1I+S13Y, T1I+T22P, T1I+S25P, T1I+S27L, T1I+S39P, T1I+S42G, T1I+S42A, T1I+S42T, T1I+S57W, T1I+S57Y, T1I+S57F, T1I+S59V, T1I+S59I, T1I+S59L, T1I+V76L, T1I+V76I, T1I+Q109R, T1I+S116D, T1I+S116E, T1I+T127V, T1I+T127I, T1I+T127L, T1I+S144P, T1I+A147H, T1I+S167L, T1I+S167I, T1I+S167V, T1I+G175D, T1I+G175E, T1L+S13Y, T1L+T22P, T1L+S25P, T1L+S27L, T1L+S39P, T1L+S42G, T1L+S42A, T1L+S42T, T1L+S57W, T1L+S57Y, T1L+S57F, T1L+S59V, T1L+S59I, T1L+S59L, T1L+V76L, T1L+V76I, T1L+Q109R, T1L+S116D, T1L+S116E, T1L+T127V, T1L+T127I, T1L+T127L, T1L+S144P, T1L+A147H, T1L+S167L, T1L+5167I, T1L+S167V, T1L+G175D, T1L+G175E, T1V+S13Y, T1V+T22P, T1V+S25P, T1V+S27L, T1V+S39P, T1V+S42G, T1V+S42A, T1V+S42T, T1V+S57W, T1V+S57Y, T1V+S57F, T1V+S59V, T1V+559I, T1V+S59L, T1V+V76L, T1V+V76I, T1V+Q109R, T1V+S116D, T1V+S116E, T1V+T127V, T1V+T127I, T1V+T127L, T1V+S144P, T1V+A147H, T1V+S167L, T1V+5167I, T1V+S167V, T1V+G175D, T1V+G175E, S13Y+T22P, S13Y+S25P, S13Y+S27L, S13Y+S39P, S13Y+S42G, S13Y+S42A, S13Y+S42T, S13Y+S57W, S13Y+S57Y, S13Y+S57F, S13Y+S59V, S13Y+559I, S13Y+S59L, S13Y+V76L, S13Y+V76I, S13Y+Q109R, S13Y+S116D, S13Y+S116E, S13Y+T127V, S13Y+T127I, S13Y+T127L, S13Y+S144P, S13Y+A147H, S13Y+S167L, S13Y+5167I, S13Y+S167V, S13Y+G175D, S13Y+G175E, T22P+S25P, T22P+S27L, T22P+S39P, T22P+S42G, T22P+S42A, T22P+S42T, T22P+S57W, T22P+S57Y, T22P+S57F, T22P+S59V, T22P+S59I, T22P+S59L, T22P+V76L, T22P+V76I, T22P+Q109R, T22P+S116D, T22P+S116E, T22P+T127V, T22P+T127I, T22P+T127L, T22P+S144P, T22P+A147H, T22P+S167L, T22P+S167I, T22P+S167V, T22P+G175D, T22P+G175E, S25P+S27L, S25P+S39P, S25P+S42G, S25P+S42A, S25P+S42T, S25P+S57W, S25P+S57Y, S25P+S57F, S25P+S59V, S25P+S59I, S25P+S59L, S25P+V76L, S25P+V76I, S25P+Q109R, S25P+S116D, S25P+S116E, S25P+T127V, S25P+T127I, S25P+T127L, S25P+S144P, S25P+A147H, S25P+S167L, S25P+S167I, S25P+S167V, S25P+G175D, S25P+G175E, S27L+S39P, S27L+S42G, S27L+S42A, S27L+S42T, S27L+S57W, S27L+S57Y, S27L+S57F, S27L+S59V, S27L+S59I, S27L+S59L, S27L+V76L, S27L+V76I, S27L+Q109R, S27L+S116D, S27L+S116E, S27L+T127V, S27L+T127I, S27L+T127L, S27L+S144P, S27L+A147H, S27L+S167L, S27L+S167I, S27L+S167V, S27L+G175D, S27L+G175E, S39P+S42G, S39P+S42A, S39P+S42T, S39P+S57W, S39P+S57Y, S39P+S57F, S39P+S59V, S39P+S59I, S39P+S59L, S39P+V76L, S39P+V76I, S39P+Q109R, S39P+S116D, S39P+S116E, S39P+T127V, S39P+T127I, S39P+T127L, S39P+S144P, S39P+A147H, S39P+S167L, S39P+S167I, S39P+S167V, S39P+G175D, S39P+G175E, S42G+S57W, S42G+S57Y, S42G+S57F, S42G+S59V, S42G+S59I, S42G+S59L, S42G+V76L, S42G+V76I, S42G+Q109R, S42G+S116D, S42G+S116E, S42G+T127V, S42G+T127I, S42G+T127L, S42G+S144P, S42G+A147H, S42G+S167L, S42G+S167I, S42G+S167V, S42G+G175D, S42G+G175E, S42A+S57W, S42A+S57Y, S42A+S57F, S42A+S59V, S42A+S59I, S42A+S59L, S42A+V76L, S42A+V76I, S42A+Q109R, S42A+S116D, S42A+S116E, S42A+T127V, S42A+T127I, S42A+T127L, S42A+S144P, S42A+A147H, S42A+S167L, S42A+S167I, S42A+S167V, S42A+G175D, S42A+G175E, S42T+S57W, S42T+S57Y, S42T+S57F, S42T+S59V, S42T+S59I, S42T+S59L, S42T+V76L, S42T+V76I, S42T+Q109R, S42T+S116D, S42T+S116E, S42T+T127V, S42T+T127I, S42T+T127L, S42T+S144P, S42T+A147H, S42T+S167L, S42T+S167I, S42T+S167V, S42T+G175D, S42T+G175E, S57W+S59V, S57W+S59I, S57W+S59L, S57W+V76L, S57W+V76I, S57W+Q109R, S57W+S116D, S57W+S116E, S57W+T127V, S57W+T127I, S57W+T127L, S57W+S144P, S57W+A147H, S57W+S167L, S57W+S167I, S57W+S167V, S57W+G175D, S57W+G175E, S57Y+S59V, S57Y+S59I, S57Y+S59L, S57Y+V76L, S57Y+V76I, S57Y+Q109R, S57Y+S116D, S57Y+S116E, S57Y+T127V, S57Y+T127I, S57Y+T127L, S57Y+S144P, S57Y+A147H, S57Y+S167L, S57Y+S167I, S57Y+S167V, S57Y+G175D, S57Y+G175E, S57F+S59V, S57F+S59I, S57F+S59L, S57F+V76L, S57F+V76I, S57F+Q109R, S57F+S116D, S57F+S116E, S57F+T127V, S57F+T127I, S57F+T127L, S57F+S144P, S57F+A147H, S57F+S167L, S57F+S167I, S57F+S167V, S57F+G175D, S57F+G175E, S59V+V76L, S59V+V76I, S59V+Q109R, S59V+S116D, S59V+S116E, S59V+T127V, S59V+T127I, S59V+T127L, S59V+S144P, S59V+A147H, S59V+S167L, S59V+S167I, S59V+S167V, S59V+G175D, S59V+G175E, S59I+V76L, S59I+V76I, S59I+Q109R, S59I+S116D, S59I+S116E, S59I+T127V, S59I+T127I, S59I+T127L, S59I+S144P, S59I+A147H, S59I+S167L, S59I+S167I, S59I+S167V, S59I+G175D, S59I+G175E, S59L+V76L, S59L+V76I, S59L+Q109R, S59L+S116D, S59L+S116E, S59L+T127V, S59L+T127I, S59L+T127L, S59L+S144P, S59L+A147H, S59L+S167L, S59L+S167I, S59L+S167V, S59L+G175D, S59L+G175E, V76L+Q109R, V76L+S116D, V76L+S116E, V76L+T127V, V76L+T127I, V76L+T127L, V76L+S144P, V76L+A147H, V76L+S167L, V76L+S167I, V76L+S167V, V76L+G175D, V76L+G175E, V76I+Q109R, V76I+S116D, V76I+S116E, V76I+T127V, V76I+T127I, V76I+T127L, V76I+S144P, V76I+A147H, V76I+S167L, V76I+S167I, V76I+S167V, V76I+G175D, V76I+G175E, Q109R+S116D, Q109R+S116E, Q109R+T127V, Q109R+T127I, Q109R+T127L, Q109R+S144P, Q109R+A147H, Q109R+S167L, Q109R+S167I, Q109R+S167V, Q109R+G175D, Q109R+G175E, S116D+T127V, S116D+T127I, S116D+T127L, S116D+S144P, S116D+A147H, S116D+S167L, S116D+S167I, S116D+S167V, S116D+G175D, S116D+G175E, S116E+T127V, S116E+T127I, S116E+T127L, S116E+S144P, S116E+A147H, S116E+S167L, S116E+5167I, S116E+S167V, S116E+G175D, S116E+G175E, T127V+S144P, T127V+A147H, T127V+S167L, T127V+S167I, T127V+S167V, T127V+G175D, T127V+G175E, T127I+S144P, T127I+A147H, T127I+S167L, T127I+51671, T127I+S167V, T127I+G175D, T127I+G175E, T127L+S144P, T127L+A147H, T127L+S167L, T127L+S167I, T127L+S167V, T127L+G175D, T127L+G175E, S144P+A147H, S144P+S167L, S144P+S167I, S144P+S167V, S144P+G175D, S144P+G175E, A147H+S167L, A147H+S167I, A147H+S167V, A147H+G175D, A147H+G175E, S167L+G175D, S167L+G175E, 5167I+G175D, 5167I+G175E, S167V+G175D and S167V+G175E.

4. The DNase variant according to claim 1 , wherein the two or more mutations are selected from the group consisting of T1I, S13Y, T22P, S25P, S27L, S39P, S42G, S57W, S59V, V76L, Q109R, S116D, T127V, S144P, A147H, S167L and G175D.

5. The DNase variant according to claim 1 , wherein the variant comprises at least three substitutions selected from the group consisting of: T1I, T22P, S57W, V76L, A147H, S167L and G175D.

6. The DNase variant according to claim 5 , wherein the DNase variant is selected from the group consisting of:

a) T1I+T22P+S57W+V76L+A147H+S167L,

b) T1I+T22P+S57W+V76L+A147H+G175D,

c) T1I+T22P+S57W+V76L+S167L+G175D,

d) T1I+T22P+S57W+A147H+S167L+G175D,

e) T1I+T22P+V76L+A147H+S167L+G175D,

f) T1I+S57W+V76L+A147H+S167L+G175D,

g) T22P+S57W+V76L+A147H+S167L+G175D, and

h) T1I+T22P+S57W+V76L+A147H+S167L+G175D.

7. The DNase variant according to claim 1 , wherein the variant comprises 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 or all of the mutations selected from the group consisting of: T1I, S13Y, T22P, S25P, S27L, S39P, S42G, S57W, S59V, V76L, Q109R, S116D, T127V, S144P, A147H, S167L and G175D.

8. The DNase variant of claim 1 , wherein the variant further comprises one or more of the substitutions selected from the group consisting of: G4N, T5F, T5C, P6V, P6G, S7D, S7T, K8V, S9K, S9Q, S9V, S9L, S9F, S9P, S9R, A10D, A10M, A10I, A10Q, A10V, A10L, A10K, Q12S, Q12V, Q12E, Q14M, Q14R, N16S, A17C, A17V, A17E, A17T, T19K, T19L, T19S, T191, T19V, K21E, K21M, G24Y, S27R, G28L, Y29W, S30K, S30D, S30H, S30T, D32Q, L33K, 138V, 138M, Q40V, V49R, L511, K521, K52H, A55S, D56I, D56L, D56T, Y58A, Y58T, N61D, P63A, T65L, T651, T65V, T65R, T65K, S66W, S66Y, S68V, S681, S68W, S68Y, S68H, S68C, S68T, S68L, T77N, T77Y, T77W, T77R, F781, F78H, F78Y, F78C, T79G, T79R, N80K, S82L, S82E, S82K, S82R, S82H, D83C, D83F, D83L, L92T, A93G, E94N, G99S, S101D, S101A, S102M, S102L, S102V, S102A, S102K, S102T, S102R, T104P, T104A, T105V, T1051, S106L K107L, K107C, K107R, K107H, K107S, K107M, K107E, K107A, K107D, A112S, A125K, S1261, S126E, S126A, S126C, S130A S130E, G132R, D135R, T138Q, W139R, R143E, R143K, G145V, G145E, G145D, G145A, G149S, K152H, K152R, S156C, S156G, S156K, S156R, S156T, S156A, T157S, Y159F, K160V, W161L, W161Y, G162Q, G162D, G162M, G162R, G162A, G162S, G162E, G162L, G162K, G162V, G162H, S164R, S164T, Q166D, S168V, S168E, S168D, S168L, K170S, K170L, K170F, K170R, T171D, T171E, T171A, T171C, A172G, A172S, L173T, L173A, L173V, Q174L, M176H, L1771, N178D, N178E, N178T, N178S, N178A, S179E, S181R, S181E, S181D, S181F, S181H, S181W, S181L, S181M, S181Y, S181Q, S181G, S181A, Y182M, Y182C, Y182K, Y182G, Y182A, Y182S, Y182V, Y182D, Y182Q, Y182F, Y182L, Y182N, Y1821, Y182E, Y182T and Y182W.

9. The DNase variant according to claim 8 , wherein the DNase variant is selected from the group consisting of:

a) T1I+S13Y+T22P+S25P+S27L+S39P+S42G+S57W+S59V+V76L+S144P+A147H+S167L+G175D;

b) T1I+S13Y+T22P+S27L+S42G+S57W+S59V+V76L+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

c) T1I+S13Y+T22P+S25P+S27L+S39P+S42G+S57W+S59V+V76L+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

d) T1I+S13Y+T22P+S25P+S27L+S39P+S42G+S57W+S59V+V76L+T77Y+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

e) T1I+T22P+D56I+S57W+V76L+Q109R+S116D+A147H+G162S+S167L+G175N;

f) T1I+S13Y+T22P+S27L+S39P+S42G+D56I+S57W+S59V+V76L+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

g) T1I+T22P+S25P+S27L+S42G+D56I+S57Y+S59V+V76L+T77Y+Q109R+S116D+T127V+S144P+A147H+Q166D+S167L+G175D+S181L;

h) T1I+S13Y+T22P+S25P+S27L+S39P+S42G+D56I+S57W+S59V+V76L+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

i) T1I+S13Y+T22P+S25P+S27L+S39P+S42G+D56I+S57W+S59V+V76L+Q109R+S116D+T127V+S144P+A147H+Q166D+S167L+G175D;

j) T1I+S13Y+T22P+S27R+S39P+S42G+D56I+S57W+S59V+V76L+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

k) T1I+S13Y+T22P+S27L+S39P+S42G+D56I+S57W+S59V+T65L+V76L+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

l) T1I+S13Y+T22P+S27L+L33K+S39P+S42G+D56I+S57W+S59V+T65V+V76L+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

m) T1I+S13Y+T22P+S25P+S27R+S39P+S42G+S57W+S59V+S66W+V76L+T77Y+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

n) T1I+S13Y+T22P+S25P+S27L+L33K+S39P+S42G+S57W+S59V+T65V+V76L+T77Y+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

o) T1I+S13Y+T22P+S25P+S27L+L33K+S39P+S42G+S57W+S59V+S66Y+V76L+T77Y+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

p) T1I+S13Y+T22P+S25P+S27L+S39P+S42G+S57W+S59V+T65V+S66Y+V76L+T77Y+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

q) T1I+S13Y+T22P+S27L+S39P+S42G+D56L+S57W+S59V+T65V+V76L+Q109R+S116D+T127V+S144P+A147H+G162D+S167L+G175D;

r) T1I+S13Y+T22P+S27R+S39P+S42G+D56L+S57W+S59V+T65V+V76L+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

s) T1I+S13Y+T22P+S27R+S39P+S42G+D56L+S57W+S59V+T65V+V76L+Q109R+S116D+T127V+S144P+A147H+G162D+S167L+G175D;

t) T1I+S13Y+T22P+S27K+S39P+S42G+D56I+S57W+S59V+T65V+V76L+S106L+Q109R+S116D+T127V+S144P+A147H+S167L+G175D;

u) T1I+S13Y+T22P+S27K+S39P+S42G+D56I+S57W+S59V+T65V+V76L+Q109R+S116D+T127V+S130A+S144P+A147H+S167L+G175D;

v) T1I+S13Y+T22P+S27L+S39P+S42G+D56L+S57W+S59V+T65L+V76L+Q109R+S116D+T127V+S130A+S144P+A147H+S167L+G175D; and

w) T1I+S13Y+T22P+S27L+S39P+S42G+D56I+S57W+S59V+T65V+V76L+Q109R+S116D+T127V+S144P+A147H+G162D+S167L+G175.

10. The DNase variant according to claim 1 , wherein the variant comprises one or both motifs [D/M/L][S/T]GYSR[D/N] (SEQ ID NO: 25) or ASXNRSKG (SEQ ID NO: 26).