Tumor tissue cryopreservation kit, tumor tissue resuscitation kit, and method of cryopreserving and/or resuscitating tumor tissue
A kit, including: a first solution, a second solution, and a metal mesh. The first solution includes: dulbecco's modified eagle medium (DMEM), 65-95 V/V %; dimethyl sulfoxide (DMSO), 5.5-20 V/V %; ethylene glycol (EG), 3.5-15 V/V %; bovine serum albumin (BSA), 0.5-4 W/V %; sucrose 1-5 W/V %; methylcellulose with a viscosity of 4000 centipoise (cP), 0.05-0.8 W/V %; hetastarch 0.25-0.6 W/V %; and glucose 15-35 W/V %. The second solution includes: DMEM, 65-95 V/V %; DMSO, 5.5-20 V/V %; EG, 8-20 V/V %; BSA, 0.5-4 W/V %; sucrose, 10-20 W/V %; methylcellulose with a viscosity of 4000 centipoise (cP), 0.05-0.8 W/V %; polyvinyl pyrrolidone (PVP), 0.25-0.6 W/V %; and glucose 15-35 W/V %. The metal mesh has a thickness of 0.15-0.2 mm and includes a plurality of square holes. The side length of the square holes is 2.0-3.0 mm, and the spacing between adjacent holes is 0.5-2.0 mm.
1. A kit, comprising:
a first solution, comprising: dulbecco's modified eagle medium (DMEM), 65-95 V/V %; dimethyl sulfoxide (DMSO), 5.5-20 V/V %; ethylene glycol (EG), 3.5-15 V/V %; bovine serum albumin (BSA), 0.5-4 W/V %; sucrose 1-5 W/V %; methylcellulose with a viscosity of 4000 centipoise (cP), 0.05-0.8 W/V %; hetastarch 0.25-0.6 W/V %; glucose 15-35 W/V %;
a second solution, comprising: DMEM, 65-95 V/V %; DMSO, 5.5-20 V/V %; EG, 8-20 V/V %; BSA, 0.5-4 W/V %; sucrose, 10-20 W/V %; methylcellulose with a viscosity of 4000 centipoise (cP), 0.05-0.8 W/V %; polyvinyl pyrrolidone (PVP), 0.25-0.6 W/V %; glucose 15-35 W/V %; and
a metal mesh, with a thickness of 0.15-0.2 mm, and comprising a plurality of square holes, a side length of the square holes being 2.0-3.0 mm, and a spacing between adjacent holes being 0.5-2.0 mm.
2. The kit of claim 1 , wherein:
the first solution comprises: dulbecco's modified eagle medium (DMEM), 80 V/V %; dimethyl sulfoxide (DMSO), 10 V/V %; ethylene glycol (EG), 10 V/V %; bovine serum albumin (BSA), 3 W/V %; sucrose 1 W/V %; methylcellulose with a viscosity of 4000 centipoise (cP), 0.05 W/V %; hetastarch 0.25 W/V %; glucose 25 W/V %; and
the second solution comprises: DMEM, 70 V/V %; DMSO, 18 V/V %; EG, 12 V/V %; BSA, 3 W/V %; sucrose, 20 W/V %; methylcellulose with a viscosity of 4000 centipoise (cP), 0.05 W/V %; PVP, 0.25 W/V %; glucose 30 W/V %.
3. The kit of claim 1 , further comprising a slicer; wherein the slicer comprises a pedestal; the pedestal comprises an upper surface, a recessed portion disposed on the upper surface, and a plurality of guiding grooves which are equidistantly distributed and vertically downward from the upper surface of the pedestal; and a depth of the plurality of guiding grooves is greater than a depth of the recessed portion.
4. The kit of claim 3 , wherein the depth of the plurality of guiding grooves is greater than the depth of the recessed portion by 1.8-2.3 mm, and a spacing between two adjacent guiding grooves is 1 mm.
5. The kit of claim 3 , wherein the recessed portion is an ellipsoid with a width of 16 mm and a length of 25 mm; and a vertical distance from a lowest point of the recessed poriton to the upper surface of the pedestal is 8.5 mm.