Use of term amniotic fluid cells for the treatment of acute and chronic respiratory diseases
Methods for purifying, culturing and selecting mesenchymal stem cell (MSC) subpopulations with neonatal quality and adult tissue specificity are for in production of advanced therapeutic medicinal products. In certain examples, term amniotic fluid derived cells having characteristics of lung cells can be isolated. In some the term amniotic fluid derived cells having characteristics of lung cells can be used in the treatment of acute and chronic respiratory diseases.
1. A method of treating acute respiratory distress syndrome in a patient in need thereof, comprising:
performing adherence selection on term amniotic fluid (TAF) cells to obtain TAF adherence cells;
passaging the TAF adherence cells to obtain TAF mesenchymal stem cells (TAF MSCs);
selecting TAF MSCs that express Endosialin to obtain lung TAF MSCs; and
administering the lung TAF MSCs to the patient.
2. The method of claim 1 , wherein selecting TAF MSCs to obtain Lung TAF MSCs further comprises selecting TAF MSCs that express DDR1.
3. The method of claim 1 , wherein selecting TAF MSCs to obtain Lung TAF MSCs further comprises selecting TAF MSCs that express LRRC38.
4. The method of claim 1 , wherein selecting TAF MSCs to obtain lung TAF MSCs further comprises selecting TAF MSCs that express a Group A surface marker selected from the group consisting of PCDH19, DDR1, MME, IFITM10, BGN, NOTCH3, SULF1, TNFSF18, BDKRB1, FLT1, PDGFRA, TNFSF4, UNC5B, FAP, CASP1, CD248, DDR2, PCDH18, LRRC38, and CRLF1.
5. The method of claim 1 , wherein selecting lung TAF MSCs further comprises excluding TAF MSCs that express a Group B surface marker selected from the group consisting of CD24, ITGB4, TNFSF10, GFRA1, CD74, FGFR4, HAVCR1, and OSCAR.
6. The method of claim 1 , wherein the lung TAF MSCs are administered at a concentration of about 1-2 million cells per kg of body weight of the patient.
7. The method of claim 1 , wherein administering the lung TAF MSCs comprises administering the lung TAF MSCs via an intravenous drip.
8. The method of claim 7 , wherein the intravenous drip comprises about 40 drops per minute.