Compositions and methods of making expanded hematopoietic stem cells using derivatives of fluorene
This invention is directed to, inter alia, compounds, methods, systems, and compositions for the maintenance, enhancement, and expansion of hematopoietic stem cells derived from one or more sources of CD34+ cells. Sources of CD34+ cells include bone marrow, cord blood, mobilized peripheral blood, and non-mobilized peripheral blood. Also provided herein are compounds of Formula I which are useful in maintaining, enhancing, and expanding of hematopoietic stem cells.
1. A compound of Formula IIIa1
or a pharmaceutically acceptable salt, hydrate, or solvate thereof; wherein,
R 1 is —NH—C(O)—R 1a NR b —C(O)—R 1a ,
R 1a is selected from the group consisting of C 2-6 alkyl and C 2-6 haloalkyl;
R 2 is —OH; and
the subscript n is 0 or 1.
2. The compound of claim 1 , wherein n is 0.
3. The compound of claim 1 , wherein n is 1.
4. The compound of claim 1 , wherein R 1a is C 2-6 haloalkyl.
5. The compound of claim 1 , wherein R 1a is C 2-6 alkyl.
6. The compound of claim 1 , wherein n is 0 and R 1a is C 2-4 alkyl.
7. The compound of claim 1 , wherein n is 0 and R 1a is C 2-4 haloalkyl.
8. A compound of claim 1 , having the structure
9. A compound of claim 1 , having the structure
or a pharmaceutically acceptable salt thereof.
10. A compound of claim 1 , having the structure
11. A compound of claim 1 , having the structure
or a pharmaceutically acceptable salt thereof.
12. A compound of claim 1 , having the structure
13. A compound of claim 1 , having the structure
or a pharmaceutically acceptable salt thereof.
14. The compound of claim 1 , wherein said compound is selected from the group consisting of