Method for enrichment and expansion of virus antigen-specific T cells
The present invention relates to a method for inducing and proliferating target virus antigen-specific dual activated T cells, and can produce target virus antigen-specific dual activated T cells by treating monocytes, which are isolated from peripheral blood, with a cytokine and a virus antigen peptide mixture and culturing the same.
1. A method for inducing and proliferating virus antigen-specific dual activated T cells comprising:
culturing peripheral blood mononuclear cells under IFN-γ and a virus antigen peptide mixture for 4 days to selectively induce virus antigen-specific dual activated T cells; and
proliferating the virus antigen-specific dual activated T cells under the presence of IL-2 and in the absence of anti-CD3 antibodies after the culturing step,
wherein the proliferated virus antigen-specific dual activated T cells comprise CD3+CD8+CD56-cells and CD3+CD8+CD56+cells.
2. The method of claim 1 , wherein the peripheral blood mononuclear cells are obtained from a normal person or a patient.
3. The method of claim 1 , wherein the IFN-γ is used at a concentration of 500 U/mL to 1,000 U/mL.
4. The method of claim 1 , wherein the IL-2 is used at a concentration of 200 U/mL to 1,000 U/mL.
5. The method of claim 1 , wherein the induction and proliferation of the dual activated T cells is carried out for a total of 20 days or more.
6. The method of claim 5 , wherein the induction and proliferation of the dual activated T cells is carried out for a total of 21 days.
7. The method of claim 1 , wherein the virus antigen comprises antigens selected from the group consisting of a cytomegalovirus (CMV), Epstein-Barr virus (EBV), an adenovirus (ADV), and BK virus (BKV).