Animal feed additives and compositions comprising an S8 serine protease
The present invention relates to isolated polypeptides having protease activity, animal feed additives comprising said polypeptides improving the nutritional value of an animal feed by adding the protease, invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides.
1. An animal feed additive comprising
(a) a polypeptide having protease activity, wherein the polypeptide has at least 98% sequence identity to amino acids 26-425 of SEQ ID NO: 1 or wherein the polypeptide is a fragment of amino acids 26-425, wherein the polypeptide optionally further comprises an N-terminal and/or C-terminal His-tag and/or HQ-tag and/or the polypeptide optionally further comprises an N-terminal and/or C-terminal extension of up to 10 amino acids; and one or more of
(b) a fat-soluble vitamin,
(c) a water-soluble vitamin, and
(d) a trace mineral.
2. The animal feed additive of claim 1 , which comprises a fat-soluble vitamin, a water-soluble vitamin, and a trace mineral.
3. The animal feed additive of claim 1 , wherein the polypeptide has at least 98% sequence identity to amino acids 26-425 of SEQ ID NO: 1.
4. The animal feed additive of claim 1 , wherein the polypeptide has at least 99% sequence identity to amino acids 26-425 of SEQ ID NO: 1.
5. The animal feed additive of claim 1 , wherein the polypeptide is amino acids 26-425 of SEQ ID NO: 1.
6. The animal feed additive of claim 1 , wherein the polypeptide is a fragment of amino acids 26-425 of SEQ ID NO: 1, wherein the fragment has protease activity.
7. The animal feed additive of claim 1 , wherein the polypeptide further comprises an N-terminal and/or C-terminal His-tag and/or HQ-tag.
8. The animal feed additive of claim 1 , wherein the polypeptide further comprises an N-terminal and/or C-terminal extension of up to 10 amino acids.
9. The animal feed additive of claim 1 , which further comprises one or more amylases; phytases; xylanases; galactanases; alpha-galactosidases; proteases, phospholipases, beta-glucanases, or any mixture thereof.
10. An animal feed comprising the animal feed additive of claim 1 .
11. The animal feed of claim 10 , which further comprises a vegetable protein.
12. The animal feed of claim 10 , which further comprises lupine, pea, or bean.
13. The animal feed of claim 10 , which further comprises soybean meal.
14. The animal feed of claim 10 , which further comprises beet, sugar beet, spinach or quinoa.
15. The animal feed of claim 10 , which further comprises soybean.
16. The animal feed of claim 10 , which further comprises barley, wheat, rye, oat, maize, rice, or sorghum.
17. The animal feed of claim 10 , which further comprises a eubiotic.
18. The animal feed of claim 10 , which further comprises a probiotic.
19. The animal feed of claim 10 , which further comprises a prebiotic.
20. A method of improving the digestibility of protein in an animal, comprising feeding the animal with the animal feed of claim 10 .