Flow control and processing cartridge

1. A flow control and processing cartridge used in a nucleic acid analysis apparatus, comprising:

a cartridge body comprising plural first chambers and plural first channels connected with the plural first chambers for storing and processing at least one of at least one sample, at least one reagent, and at least one buffer and configured to perform at least one of sample purification and nucleic acid extraction; and

a reaction chip in conjunction with the cartridge body and comprising:

plural second chambers and plural second channels connected with the plural second chambers configured to store and process at least one amplification reaction solution; and

at least two fluidic networks configured to perform nucleic acid amplification and detection, wherein at least one of the fluidic networks comprises plural detection wells, a main fluid channel connected with the detection wells and configured to dispense the sample or control liquids into the detection wells, and a gas releasing channel connected with the detection wells and configured to release gas from the detection wells, wherein one of the fluidic networks is configured for quality control.

2. The flow control and processing cartridge according to claim 1 , wherein the control liquids include a positive control liquid and a negative control liquid.

3. The flow control and processing cartridge according to claim 1 , wherein parts of the second chambers and parts of the second channels of the reaction chip jointly form a metering unit.

4. The flow control and processing cartridge according to claim 3 , wherein the metering unit comprises a storage chamber, at least one metering chamber, an overflow chamber, and channels connecting the storage chamber, the metering chamber, and the overflow chamber.

5. The flow control and processing cartridge according to claim 1 , wherein parts of the second chambers and parts of the second channels of the reaction chip jointly form a mixing and debubbling unit.

6. The flow control and processing cartridge according to claim 5 , wherein the mixing and debubbling unit comprises a storage chamber, a mixing chamber, a mixture chamber, a ball contained in the mixing chamber, and channels connecting the storage chamber, the mixing chamber, and the mixture chamber.

7. The flow control and processing cartridge according to claim 6 , wherein the mixing chamber has a bottom hole, and the ball lays on the bottom hole as a single-direction valve to block the bottom hole when the at least one amplification reaction solution is delivered into the mixing chamber.

8. The flow control and processing cartridge according to claim 6 , wherein the ball is made of or coated with a layer of a biocompatible material.

9. The flow control and processing cartridge according to claim 6 , wherein a mixing function of the mixing and debubbling unit is implemented by pumping air into the amplification reaction solution contained in the mixing chamber to agitate the amplification reaction solution.

10. The flow control and processing cartridge according to claim 6 , wherein a debubbling function of the mixing and debubbling unit is implemented by pumping air into the mixing chamber to pressurize the air and break bubbles in the amplification reaction solution.

11. The flow control and processing cartridge according to claim 1 further comprising a thermal treatment chip in conjunction with the cartridge body.

12. The flow control and processing cartridge according to claim 1 , wherein the gas releasing channel is much narrower than the main fluid channel.

13. The flow control and processing cartridge according to claim 1 , wherein the main fluid channel comprises plural wide channel parts, plural narrow channel parts, and plural well inlet channels.

14. The flow control and processing cartridge according to claim 13 , wherein each of the plural wide channel parts is aligned with one of the plural detection wells and connected with the corresponding detection well through the corresponding well inlet channel, and each of the plural narrow channel parts is connected between two adjacent wide channel parts.

15. The flow control and processing cartridge according to claim 14 , wherein a flow resistance at the narrow channel part is higher than a combined flow resistance at the wide channel part and at the well inlet channel.

16. The flow control and processing cartridge according to claim 14 , wherein the well inlet channel has lower flow resistance on one end connected with the detection well and higher flow resistance on the other end far away from the detection well.

17. The flow control and processing cartridge according to claim 1 , wherein the reaction chip comprises a planar portion and a column portion, and the column portion is extended from a bottom of the planar portion and has a smaller cross-section than the planar portion.

18. The flow control and processing cartridge according to claim 1 , wherein the reaction chip further comprises at least one sample loading port for adding the sample into the cartridge.

19. The flow control and processing cartridge according to claim 1 , wherein the reaction chip further comprises plural sample loading ports for adding different samples into the cartridge.

20. The flow control and processing cartridge according to claim 1 , wherein the detection well comprises at least one light transmissive thin wall or thin film for passing light therethrough.