Bacterial secretome for use in the treatment of skin lesions
The present invention relates to a novel use of a bacterial secretome in the field of the treatment of skin lesions and more particularly of wound healing. The invention also relates to cosmetic or dermatological compositions comprising such a bacterial secretome as active agent.
1. A method of treatment of skin lesions which comprises administering to a patient in need thereof a bacterial secretome of a non-pathogenic Gram-negative bacterium belonging to the class Betaproteobacteria, subfamily Neisseriaceae, wherein the bacterium is the bacterium deposited with the CNCM on 8 Apr. 2010 under number 14920, and
said secretome is obtained by a process comprising the steps of:
a) culturing said bacterium in a culture medium and under conditions suitable for its growth,
b) liquid/solid separation of the culture medium and recovery of the liquid phase,
c) adding a basic buffer to the liquid phase obtained in step b) and incubating the buffered liquid phase obtained for 1 to 7 hours, optionally at a temperature between 1 and 6° C., and
d) obtaining said secretome comprising biomolecules secreted by said bacterium in the liquid phase.
2. The method according to claim 1 , wherein the basic buffer is selected from Tris buffer, arginine buffer and mixtures thereof.
3. The method according to claim 1 , wherein step b) of liquid/solid separation is followed by a step b′) of clarification of the liquid phase or of the buffered liquid phase by filtration before step c).
4. The method according to claim 3 , wherein the filtration is performed with a 0.2 μm cut-off.
5. The method according to claim 1 , wherein said biomolecules comprise peptides, proteins and secondary metabolites secreted by said bacterium.
6. The method according to claim 1 , wherein the skin lesions are selected from the group consisting of abrasions, burns, sunburns, scratches, cuts, scrapes, stitches.
7. The method according to claim 1 , wherein the skin lesions follow trauma, surgery, a dermatological procedure, or a cosmetic medicine procedure.
8. The method according to claim 1 , wherein the treatment of skin lesions comprises improving healing and skin repair.
9. The method according to claim 1 , which further comprises administering to the patient a copper salt and/or a zinc salt.
10. The method according to claim 1 , wherein the bacterial secretome is contained in a dermatological composition containing the bacterial secretome and a dermatological excipient suitable for topical application, for use in the treatment of skin lesions.
11. The method according to claim 1 , wherein the bacterial secretome is contained in a dermatological composition containing the bacterial secretome, a copper salt and/or a zinc salt and a suitable dermatological excipient.
12. A method of treatment of skin lesions which comprises administering to a patient in need thereof a dermatological composition comprising a bacterial secretome of a non-pathogenic Gram-negative bacterium belonging to the class Betaproteobacteria, subfamily Neisseriaceae, a copper salt and/or a zinc salt and a suitable dermatological excipient, wherein
the bacterium is the bacterium deposited with the CNCM on 8 Apr. 2010 under number 14920, and
said secretome is obtained by a process comprising the steps of:
a) culturing said bacterium in a culture medium and under conditions suitable for its growth,
b) liquid/solid separation of the culture medium and recovery of the liquid phase,
c) adding a basic buffer to the liquid phase obtained in step b) and incubating the buffered liquid phase obtained for 1 to 7 hours, optionally at a temperature between 1 and 6° C., and
d) obtaining said secretome comprising biomolecules secreted by said bacterium in the liquid phase.
13. The method according to claim 12 , wherein the basic buffer is selected from Tris buffer, arginine buffer and mixtures thereof.