MiRNA molecule, equivalent, antagomir, or source thereof for treating and/or diagnosing a condition and/or a disease associated with neuronal deficiency or for neuronal (re)generation
The invention relates to the diagnostic and therapeutic uses of a miRNA molecule, an equivalent or a source thereof in a neuronal deficiency or a disease and condition associated with neuronal deficiency.
1. A method for treating, reverting, preventing, curing, and/or delaying epilepsy comprising administering to a subject in need thereof a therapeutically effective amount of an antagomir of a miRNA-135, or a source of such an antagomir or a pharmaceutical composition comprising an antagomir of a miRNA-135, or a source of such an antagomir,
wherein said miRNA-135 is a miRNA-135 molecule or a miRNA-135 isomiR, and is an oligonucleotide with a seed sequence comprising at least 6 of the 7 nucleotides of the seed sequence represented by SEQ ID NOs: 14-17, 19-42, 52-55, or is a source thereof.
2. The method according to claim 1 , wherein said miRNA-135 is a miRNA-135a molecule, a miRNA-135b molecule, an isomiR of miRNA-135a, or an isomiR of miRNA-135b.
3. The method according to claim 1 , wherein a source of a miRNA is a precursor of a miRNA and is an oligonucleotide of at least 50 nucleotides in length.
4. The method according to claim 1 ,
wherein said miRNA-135 shares at least 70% sequence identity with any one of SEQ ID NOs: 147-214, and/or
wherein said antagomir shares at least 70% sequence identity with any one of SEQ ID NOs: 242-245, 247-314,
and/or wherein said miRNA or antagomir is from 15-30 nucleotides in length,
and/or wherein said source of a miRNA is a precursor of said miRNA and shares at least 70% sequence identity with any one of SEQ ID NOs: 1-3 or 10-12.
5. The method according to claim 1 ,
wherein the amount of antagomir administered is effective to restore expression of myocyte-specific enhancer factor 2A (Mef2a).
6. The method according to claim 1 ,
wherein the amount of antagomir administered is effective to reduce seizure count and/or seizure duration.
7. The method according to claim 1 ,
wherein the amount of antagomir administered is effective to prevent, delay, or revert abnormal neuronal spine formation.
8. The method according to claim 1 , wherein the subject is a human with mesial temporal lobe epilepsy with hippocampal sclerosis.
9. A method for reducing spontaneous seizures in a subject with epilepsy comprising administering to the subject a therapeutically effective amount of an antagomir of a miRNA-135, or a source of such an antagomir or a pharmaceutical composition comprising an antagomir of a miRNA-135, or a source of such an antagomir,
wherein said miRNA-135 is a miRNA-135 molecule or a miRNA-135 isomiR, and is an oligonucleotide with a seed sequence comprising at least 6 of the 7 nucleotides of the seed sequence represented by SEQ ID NOs: 14-17, 19-42, 52-55, or is a source thereof.