Multi-technology printing system
A system for performing substrateless and/or local donor Laser Induced Forward Transfer (LIFT), comprising a reservoir comprising at least one opening and an energy source configured to deliver energy to a donor material within said reservoir, characterized by at least one of: said reservoir is embedded into a medical device; said reservoir is in fluid connection with a medical device; said reservoir is incorporated into a medical device; said reservoir contains at least one biologically active substance; and, said reservoir is in fluid connection with at least one source of at least one biologically active substance. This system enables deposition of material by LIFT without any need for a donor substrate. Methods of substrateless and local donor LIFT, in particular for medical and biological applications, are also disclosed.
1. A method of LIFT-based bio-printing, comprising:
introducing a quantity of a biological material into a reservoir comprising at least one opening;
placing an acceptor substrate opposite to said opening;
providing an energy source disposed to provide energy to said biological material;
applying at least one pulse of energy from said energy source to said biological material, thereby providing local heating to said biological material sufficient to create a bubble within said biological material and thereby forcing a portion of said biological material from said reservoir via said opening onto said acceptor substrate;
repeating the previous step until a bio-printed material of a predetermined structure and shape is obtained;
consolidating and stiffening said bio-printed material; and,
allowing said bio-printed material to mature.
2. The method according to claim 1 , wherein said method does not comprise any step involving the use of a donor substrate.
3. The method according to claim 1 , comprising replenishing said biological material from a material feeder in fluid connection with said reservoir.
4. The method according to claim 1 , wherein said step of introducing a quantity of a biological material into a reservoir comprising at least one opening comprises introducing a plurality of biological materials from a plurality of reservoirs.
5. The method according to claim 1 , additionally comprising providing at least one printing head in fluid connection with said reservoir.
6. The method according to claim 5 , wherein said step of providing at least one printing head comprises incorporating said reservoir into said printing head.
7. The method according to claim 1 , wherein said step of applying at least one pulse of energy from said energy source to said biological material comprises applying at least one pulse of energy so as to provide droplets having the size of a single cell.
8. The method according to claim 1 , wherein said step of consolidating and stiffening comprises at least one technique selected from the group consisting of irradiation with visible light, irradiation with UV light, and heating.
9. The method according to claim 1 , comprising continuously flowing said biological material through said reservoir.
10. The method according to claim 1 , wherein said biological material is selected from the group consisting of bio-polymers, cells, cell culture media, soluble sacrificial materials, extra-cellular materials, growth factors, and scaffolding materials.
11. The method according to claim 1 , wherein said biological material comprises cells, said cells are bio-printed along with ink, and said step of forcing a portion of said biological material from said reservoir via said opening onto said acceptor substrate comprises depositing said cells in positions so as to create functioning tissue.
12. The method according to claim 11 , wherein said functioning tissue is selected from the group consisting of vascular tissue, nerve tissue, skin tissue, ocular tissue, liver tissue, kidney tissue, bone, and cartilage.
13. The method according to claim 11 , wherein said step of forcing a portion of said biological material from said reservoir via said opening onto said acceptor substrate comprises depositing non-cellular biological material followed by at least one step of depositing cells.
14. The method according to claim 11 , comprising depositing a plurality of biological materials in separate steps.
15. The method according to claim 1 , performed on the system according to claim 1 .
16. The method according to claim 1 , performed on a system for performing substrateless and/or local donor Laser Induced Forward Transfer (LIFT), wherein said system for performing substrateless and/or local donor LIFT comprises:
a reservoir comprising at least one opening; and,
an energy source configured to deliver energy to a donor material within said reservoir and thereby initiate a LIFT process.