Pulp regeneration compositions and methods of forming and using the same
View Patent ↗A dental tissue regenerative composition. The composition includes a combination of (1) human dental pulp stem cells and (2) at least one of human umbilical vein endothelial cells or vascular endothelial growth factor. The combination is encapsulated in a light-activated gelatin methacrylate hydrogel.
1. A dental tissue regenerative composition comprising:
a material at least partly obtained from an extracted tooth; and
a light-activated gelatin methacrylate hydrogel encapsulating the material.
2. The composition of claim 1 , further comprising a photo-initiator.
3. The composition of claim 2 , wherein the photo-initiator is present at a concentration of about 0.05% to about 0.5% (w/v).
4. The composition of claim 1 , wherein the photo-initiator is selected from the group consisting of: 1-[4-(2-hydroxyethoxy)-phenyl]-2-hydroxy-2-methyl-1-propane-1-one, azobisisobutyronitrile, benzoyl peroxide, di-tert-butyl peroxide, 2,2-dimethoxy-2-phenylacetophenone, Eosin Y, and any combination thereof.
5. The composition of claim 1 , wherein the gelatin methacrylate hydrogel is present at a concentration of about 3% to about 5% (w/v).
6. The composition of claim 1 , wherein the material obtained from the extracted tooth includes dental pulp stem cells.
7. The composition of claim 6 , wherein the material further includes at least one of umbilical vein endothelial cells or vascular endothelial growth factor.
8. The composition of claim 6 , wherein the dental pulp stem cells have been isolated from dental pulp.
9. The composition of claim 8 , wherein the dental pulp is from a mature tooth.
10. The composition of claim 1 , wherein the composition is configured to be used for pulpal revascularization, to replace infected pulpal tissues in pulpotomy, as a temporary intra canal medicament, or for regenerating pulp-like tissues in tooth root segments.
11. A method of regenerating pulp-like tissues in a tooth root segment of a tooth, the method comprising:
providing a composition comprising
a gelatin methacrylate hydrogel,
a photo-initiator, and
a material at least partly obtained from an extracted tooth, the gelatin methacrylate hydrogel and the material having been cross-linked by exposure to a UV or visible light; and
injecting the composition into the tooth root segment.
12. The method of claim 11 , wherein the photo-initiator is present in the composition at a concentration of about 0.05% to about 0.5% (w/v).
13. The method of claim 11 , wherein the photo-initiator is selected from the group consisting of: 1-[4-(2-hydroxyethoxy)-phenyl]-2-hydroxy-2-methyl-1-propane-1-one, azobisisobutyronitrile, benzoyl peroxide, di-tert-butyl peroxide, 2,2-dimethoxy-2-phenylacetophenone, Eosin Y, and any combination thereof.
14. The method of claim 11 , wherein the gelatin methacrylate hydrogel is present in the composition at a concentration of about 3% to about 5% (w/v).
15. The method of claim 11 , wherein the material obtained from the extracted tooth includes dental pulp stem cells.
16. The method of claim 15 , wherein the material further comprises at least one of umbilical vein endothelial cells or vascular endothelial growth factor.
17. The method of claim 15 , wherein the dental pulp stem cells have been isolated from dental pulp.
18. The method of claim 17 , wherein the dental pulp is from a mature tooth.
19. A method of forming a dental tissue regenerative composition, the method comprising:
combining a material at least partly obtained from an extracted tooth, a gelatin methacrylate hydrogel, and a photo-initiator; and
cross-linking the gelatin methacrylate hydrogel with the material by exposure to a UV or visible light.
20. The method of claim 19 , wherein the photo-initiator is present at a concentration of about 0.05% to about 0.5% (w/v).
21. The method of claim 19 , wherein the photo-initiator is selected from the group consisting of: 1-[4-(2-hydroxyethoxy)-phenyl]-2-hydroxy-2-methyl-1-propane-1-one, azobisisobutyronitrile, benzoyl peroxide, di-tert-butyl peroxide, 2,2-dimethoxy-2-phenylacetophenone, Eosin Y, and any combination thereof.
22. The method of claim 19 , wherein the gelatin methacrylate hydrogel is present at a concentration of about 3% to about 5% (w/v).
23. The method of claim 19 , wherein the material obtained from the extracted tooth includes dental pulp stem cells.
24. The method of claim 23 , wherein the material further comprises at least one of umbilical vein endothelial cells or vascular endothelial growth factor.
25. The method of claim 23 , wherein the dental pulp stem cells have been isolated from dental pulp.
26. The method of claim 25 , wherein the dental pulp is from a mature tooth.
27. The method of claim 19 , wherein the composition is configured to be used for pulpal revascularization, to replace infected pulpal tissues in pulpotomy, as a temporary intra canal medicament or for regenerating pulp-like tissues in tooth root segments.