Aptamers for measuring lipoprotein levels
Provided herein are aptamers for binding lipoproteins, systems for binding lipoproteins, and methods of detecting lipoproteins in a sample. The aptamers are useful for detecting the levels of lipoproteins in a biological sample and selectively detecting LDL particles in the presence of HDL particles. The aptamers can also be used as therapeutic agents against various diseases.
1. An aptamer with high affinity to LDL particles and high selectivity for LDL particles over HDL particles, wherein the aptamer includes a sequence selected from one of: SEQ. ID NO. 1: ACCTCGATTTTATATTATTTCGCTTACCAACAACTGCAGA; SEQ. ID NO. 2: GTCAGCTTCTTCTATATAATTCACCTTGCCTCTCGTTCCT; SEQ. ID NO. 3: AACCTTAATTTACCCTTGTTTTATCATTTCCATCATAGCT; SEQ. ID NO. 4:
AAACCATTCTTGTCTCGCATTTTTCTCTTTTATATTATTT; SEQ. ID NO. 5:
CACGCGCATAACCCTTTTTTCACCTGCCCTTTTAACTAAT; SEQ. ID NO. 6:
GCCATAAGATGACGACATTCATCACCAAACCAACCTCAGC; SEQ. ID NO. 7:
GCCGGGTGATTGAAAAGCAGATTACCGATCATCCAATAAA; SEQ. ID NO. 8:
AACTACATGGTATGTGGTGAACTACCTCGATTTTATATTATTTCGCTTACCAACAACT GCAGAGACGTACAATGTACCC; SEQ. ID NO. 9:
AACTACATGGTATGTGGTGAACTGTCAGCTTCTTCTATATAATTCACCTTGCCTCTCG TTCCTGACGTACAATGTACCC; SEQ. ID NO. 10:
AACTACATGGTATGTGGTGAACTAACCTTAATTTACCCTTGTTTTATCATTTCCATCA TAGCTGACGTACAATGTACCC; SEQ. ID NO. 11:
AACTACATGGTATGTGGTGAACTAAACCATTCTTGTCTCGCATTTTTCTCTTTTATAT TATTTGACGTACAATGTACCC; SEQ. ID NO. 12:
AACTACATGGTATGTGGTGAACTCACGCGCATAACCCTTTTTTCACCTGCCCTTTTAA CTAATGACGTACAATGTACCC; SEQ. ID NO. 13:
AACTACATGGTATGTGGTGAACTGCCATAAGATGACGACATTCATCACCAAACCAA CCTCAGCGACGTACAATGTACCC; or SEQ. ID NO. 14:
AACTACATGGTATGTGGTGAACTGCCGGGTGATTGAAAAGCAGATTACCGATCATCC AATAAAGACGTACAATGTACCC.
2. The aptamer of claim 1 , wherein the aptamer is linked to a surface.
3. The aptamer of claim 1 , wherein the aptamer is linked to an antisense strand that is complimentary to at least a portion of the aptamer.
4. An aptamer with high affinity to LDL particles and high selectivity for LDL particles over HDL particles, wherein the aptamer includes a sequence selected from one of: SEQ. ID NO. 10:
AACTACATGGTATGTGGTGAACTAACCTTAATTTACCCTTGTTTTATCATTTCCATCA TAGCTGACGTACAATGTACCC; SEQ. ID NO. 11:
AACTACATGGTATGTGGTGAACTAAACCATTCTTGTCTCGCATTTTTCTCTTTTATAT TATTTGACGTACAATGTACCC; SEQ. ID NO. 12:
AACTACATGGTATGTGGTGAACTCACGCGCATAACCCTTTTTTCACCTGCCCTTTTAA CTAATGACGTACAATGTACCC; SEQ. ID NO. 13:
AACTACATGGTATGTGGTGAACTGCCATAAGATGACGACATTCATCACCAAACCAA CCTCAGCGACGTACAATGTACCC; or SEQ. ID NO. 14:
AACTACATGGTATGTGGTGAACTGCCGGGTGATTGAAAAGCAGATTACCGATCATCC AATAAAGACGTACAATGTACCC.
5. The aptamer of claim 4 , wherein the aptamer is linked to a surface.
6. The aptamer of claim 4 , wherein the aptamer is linked to an antisense strand that is complimentary to at least a portion of the aptamer.