Automatic pipetting device for transferring samples and/or reagents and method for transferring liquid samples and/or reagents
Aspects of the present disclosure relate to methods and devices for automatically transferring samples and/or reagents from sample vessels and/or reagent vessels into at least one receiving vessel In one example embodiment, a pipetting device is disclosed including a pipettor that is movable along a first direction and has at least one first pipetting needle that is movable along an arm of the pipettor along a second direction, substantially normal to the first direction. The pipetting needle is lowerable along a third direction into the individual vessels. In some specific embodiments, the arm of the movable pipettor has at least one second pipetting needle which, regardless of the current position of the first pipetting needle, is movable past the first pipetting needle and is lowerable into the individual vessels.
1. An automatic pipetting device for transferring samples and/or reagents from sample vessels and/or reagent vessels into receiving vessels, the pipetting device comprising:
a rail extending in a first horizontal direction;
a pipettor movable along the rail in the first horizontal direction and including
a mount coupled to the rail and configured and arranged to move in the first horizontal direction,
a vertical tower coupled to the mount,
an arm coupled to the vertical tower and aligned in a second horizontal direction, where the second horizontal direction is substantially perpendicular to the first horizontal direction, and
at least one first pipetting needle configured and arranged to move along the arm of the pipettor in the second horizontal direction, and be lowerable, in a third direction, into the sample vessels, the reagent vessels and the receiving vessels; and
wherein the arm of the pipettor includes at least one second pipetting needle which, regardless of the current position of the first pipetting needle, is configured and arranged to be movable past the first pipetting needle and is lowerable into the sample vessels, the reagent vessels and the receiving vessels;
wherein the at least one first and the at least one second pipetting needles are arranged on opposite longitudinal sides or side faces of the arm.
2. The pipetting device according to claim 1 , wherein the at least one first pipetting needle and the at least one second pipetting needle are both hollow needles, and differ in the type of materials used, the internal volumes and/or the geometry of the hollow needles.
3. The pipetting device according to claim 1 , wherein the pipettor further includes at least one needle washing unit for the at least one first and the at least one second pipetting needles, the needle washing unit configured and arranged to be movable with the pipettor.
4. The pipetting device according to claim 1 , wherein the at least one first and the at least one second pipetting needles are configured and arranged to be movable substantially parallel to one another at least in partial regions of the arm.
5. The pipetting device according to claim 1 , wherein, on at least one side of the arm, the at least one first and the at least one second pipetting needles are connected via a lifting mechanism and are configured and arranged to be movable in opposite directions along the z-direction.
6. The pipetting device of claim 5 , wherein the at least one first and the at least one second pipetting needles are further configured and arranged to be moveable in an antiparallel manner, relative to one another.
7. The pipetting device according to claim 1 , wherein the vertical tower and the arm are substantially L-shaped.
8. The pipetting device according to claim 1 , wherein the vertical tower and the arm are substantially T-shaped.
9. A method for transferring liquid samples and/or reagents from sample vessels and/or reagent vessels into at least one receiving vessel of an automatic pipetting device according to claim 1 , comprising the following steps:
moving a first pipetting needle of the pipettor along a horizontal second direction, substantially normal to the first direction, in order to transfer a predetermined quantity of a liquid sample or reagent from a sample vessel or reagent vessel into the receiving vessel;
lowering the first pipetting needle into the sample vessel or reagent vessel,
up a predetermined quantity of sample liquid or reagent liquid and moving the first pipetting needle to the position of the receiving vessel;
dispensing the predetermined quantity of sample liquid or reagent liquid into the receiving vessel; and
moving a second pipetting needle of the same pipettor in the second direction, regardless of the current position of the first pipetting needle, in order to simultaneously carry out additional pipetting tasks.
10. The method of claim 9 , wherein the second pipetting needle is movable past the first pipetting needle and is lowerable into another sample vessel or reagent vessel.
11. The method of claim 9 , further including cleaning the first and/or second pipetting needles with a needle washing unit which is moveable with the pipettor.
12. The method of claim 9 , wherein the first pipetting needle and the second pipetting needle are movable substantially parallel to one another.
13. The pipetting device of claim 1 , wherein the at least one receiving vessel is a part of an analyzer for analyzing liquid samples.