Reaction processor
A reaction processor includes: a reaction processing vessel including a channel in which a sample moves and a pair of air communication ports, a first air communication port and a second air communication port, provided at respective ends of the channel; a temperature control system that provides a medium temperature region and a high temperature region between the first air communication port and the second air communication port in the channel; and a liquid feeding system that discharges and sucks air in order to move and stop the sample inside the channel. One of the pair of air communication ports of the reaction processing vessel that is farther away from the high temperature region communicates with the liquid feeding system via a tube. One of the pair of air communication ports of the reaction processing vessel that is closer to the high temperature region is opened to atmospheric pressure.
1. A reaction processor for applying thermal cycle to a liquid sample to cause a reaction to the sample, the reaction processor comprising:
a reaction processing vessel having for the sample to move therein, a pair of air communication ports provided at respective ends of the channel, the reaction processing vessel being detachable from the reaction processor;
a temperature control system including a heater for heating a part of the channel, the temperature control system configured to provide a first temperature region maintained at a first temperature and a second temperature region maintained at a second temperature higher than the first temperature between the pair of air communication port in the channel; and
a liquid feeding system communicating with the channel via one of the pair of air communication ports, the liquid feeding system discharging and sucking air in order to move the sample in a reciprocating manner inside the channel,
wherein the pair of air communication ports includes a first air communication port far from the second temperature region and a second air communication port close to the second temperature region,
wherein the liquid feeding system includes:
a chamber having an internal space with a constant volume, a first air vent and a second air vent allowing the internal space to communicate with the outside of the chamber;
a first pump consisting of a first micro blower with a first discharging port connected to the first vent of the chamber, the first pump installed in the internal space of the chamber; and
a second pump consisting of a second micro blower with a second discharging port connected to the second vent of the chamber, the second pump installed in the internal space of the chamber,
wherein the first air vent of the chamber communicates via a tube with the first air communication port,
wherein the second air vent of the chamber is opened to atmospheric pressure,
wherein the second air communication port is opened to atmospheric pressure,
wherein the liquid feeding system is controlled to operate the first pump and stop the second pump, the chamber sucks air from the second vent through the second pump and discharges air into the channel through the first communication port, the sample is controlled to move from the first temperature region to the second temperature region, and
wherein the liquid feeding system is controlled to operate the second pump and stop the first pump, the chamber discharges air from the second vent of the chamber and sucks air from the channel through the first communication port, the sample is controlled to move from the second temperature region to the first temperature region.
2. The reaction processor according to claim 1 , wherein the reaction processing vessel have a filter to prevent a contamination into the channel between the first air communication port and the first temperature region.
3. The reaction processor according to claim 1 , the reaction processing vessel having
a connection channel between the first temperature region and the second temperature region, further comprising:
a fluorescence detector that detects fluorescence from the sample inside the connection channel.
4. The reaction processor according to claim 3 , wherein the liquid feeding system is controlled based on a change in the fluorescence detected by the fluorescence detector.