Oxidase-based sensors and methods of using
Oxidase-based sensors and methods of using the sensors are provided.
1. A sensor for detecting an analyte, comprising one or more analyte sensing populations comprising:
(a) one or more polymers, wherein the one or more polymers is formed from one or more monomers, one or more comonomers and one or more crosslinkers;
wherein the one or more monomers are selected from the group consisting of: 2-hydroxyethyl methacrylate (HEMA) and hydroxypropyl methacrylate (HPMA); the one or more comonomers are selected from the group consisting of: HPMA and n-hexylacrylate (nHA); and the one or more crosslinkers is ethylene glycol dimethacrylate (EGDMA); wherein the one or more comonomers are is different from the one or more monomers;
(b) one or more oxidases; and
(c) one or more oxygen sensitive dyes.
2. The sensor of claim 1 , wherein the analyte is lactate and the one or more oxidases is lactate oxidase.
3. The sensor of claim 1 , wherein the analyte is glucose and the one or more oxidases is glucose oxidase.
4. The sensor of claim 1 , further comprising an oxygen reference population comprising an oxygen reference dye.
5. A method for detecting an analyte in a subject, comprising placing a sensor of claim 1 in a subject, wherein the sensor generates detectable luminescent signal.
6. The sensor of claim 1 , further comprising an additional analyte sensing population.
7. The sensor of claim 6 , wherein the additional analyte sensing population detects the analyte at a different oxygen concentration.
8. The sensor of claim 4 , wherein the oxygen reference population is between 0.1 mm and 5 mm from the one or more analyte sensing populations.
9. The sensor of claim 1 , wherein the analyte is alcohol and the one or more oxidases is alcohol oxidase.
10. The sensor of claim 1 wherein the one or more polymers are formed from HEMA monomer, HPMA comonomer, and EGDMA crosslinker.
11. The sensor of claim 10 , wherein:
the HEMA monomer is 40 wt % to 80 wt % of the one or more polymers;
the HPMA comonomer is 15 wt % to 30 wt % of the one or more polymers; and
the EGDMA is 4 wt % to 20 wt % of the one or more polymers.
12. The sensor of claim 10 , wherein:
the HEMA monomer is about 63 wt % of the one or more polymers;
the HPMA comonomer is about 27 wt % of the one or more polymers; and
the EGDMA is about 10 wt % of the one or more polymers.