Apparatus and methods for processing blood
Apparatus and methods for processing blood are disclosed in which one variation generally comprises a tube defining a channel and an access tube extending into the channel. An open cell matrix configured to entrap red blood cells may be positioned within at least a portion of the channel. Another variation generally comprises a cylindrical tube and a plunger slidably positioned within the channel. The plunger also has a funnel positioned upon the plunger and is movable therewith. Both the plunger and funnel define a fluid channel through and in communication with the cylindrical tube.
1. A method of separating components from blood, comprising:
introducing a volume of blood through a funnel and a plunger and into a channel defined by a cylindrical tube such that the funnel and the plunger are moved from a first position within the cylindrical tube to a second position where a funnel fluid opening is positioned externally of an opening defined by the cylindrical tube;
applying a centrifugal force to the volume of blood contained within the cylindrical tube such that the blood forms at least a first fractional layer and a second fractional layer; and
withdrawing at least the first fractional layer from the cylindrical tube via the funnel and the plunger such that the funnel and the plunger are moved from the second position back towards the first position away such that the funnel fluid opening is positioned within an interior of the cylindrical tube while the first fractional layer is withdrawn into the funnel through a first width and into contact with the funnel and out of the funnel fluid opening through a second width which is smaller than the first width via a vacuum force formed within the funnel.
2. The method of claim 1 further comprising introducing an anticoagulant into the cylindrical tube.
3. The method of claim 1 wherein introducing the volume of blood comprises introducing the volume of blood through a tubing attached to the funnel fluid opening.
4. The method of claim 3 further comprising removing the tubing from the funnel fluid opening prior to applying the centrifugal force.
5. The method of claim 1 wherein applying a centrifugal force comprises forming the first fractional layer comprised of a PRP layer.
6. The method of claim 1 wherein applying a centrifugal force comprises forming the second fractional layer comprised of a RBC layer.
7. The method of claim 1 wherein withdrawing at least the first fractional layer comprises suctioning the first fractional layer via a withdrawal syringe fluidly coupled to the funnel.
8. The method of claim 7 further comprising withdrawing an additional fractional layer via a second withdrawal syringe fluidly coupled to the funnel.