Kit, device and method for detecting prostate cancer
This invention provides a kit or device for detection of prostate cancer and a method for detecting prostate cancer. This invention provides a kit or device for detection of prostate cancer comprising a nucleic acid capable of specifically binding to an miRNA in a sample from a subject or a complementary strand thereof and a method for detecting prostate cancer comprising measuring the miRNA in vitro.
1. A method for detecting prostate cancer and then treating or performing a diagnostic procedure in a human subject, comprising:
measuring an expression level of has-miR-1185-1-3p in a blood, serum, or plasma sample from the subject;
comparing the measured expression level of hsa-miR-1185-1-3p to a control expression level in a sample from a healthy subject; and
detecting an increased level of hsa-miR-1185-1-3p in the sample from the subject as compared to the control expression level from the sample from the healthy subject,
wherein the increased level of hsa-miR-1185-1-3p indicates that the subject has prostate cancer, and
and further treating the subject for the prostate cancer or performing a diagnostic procedure on the subject with the prostate cancer;
wherein the treating comprises surgery, radiotherapy, chemotherapy or the combination thereof; and
wherein the diagnostic procedure comprises rectal examination, transrectal ultrasonography of the prostate, or imaging of prostate tissue.
2. The method according to claim 1 , wherein the expression level of hsa-miR-1185-1-3p in the sample is measured by using a kit comprising a nucleic acid(s) that specifically binds to hsa-miR-1185-1-3p.
3. The method according to claim 1 , wherein the expression level of hsa-miR-1185-1-3p in the sample is measured by using a device comprising a nucleic acid(s) that specifically binds to hsa-miR-1185-1-3p.
4. The method according to claim 3 , wherein the device further comprises at least one nucleic acid capable of specifically binding to at least one polynucleotide selected from the following other prostate cancer markers: miR-1185-2-3p, miR-197-5p, miR-6076, and/or miR-17-3p, miR-320b, miR-6819-5p, and miR-1228-5p.