Solution for cryopreservation of animal cells or animal tissues, cryopreserved product, and cryopreservation method
The present invention provides a solution for cryopreservation of animal cells or animal tissues which substantially includes a cryoprotectant that contains 0.5 w/v % to 6.0 w/v % of dextran or derivatives thereof or salts thereof and contains dimethyl sulfoxide, and includes a physiological aqueous solution; a cryopreserved product of animal cells or animal tissues which includes animal cells or animal tissues, and includes the above-described solution for cryopreservation of animal cells or animal tissues; and a cryopreservation method of animal cells or animal tissues, which is a method using the above-described solution for cryopreservation of animal cells or animal tissues.
1. A cryopreserved product of mesenchymal cells or mesenchymal stem cells, comprising:
mesenchymal cells or mesenchymal stem cells; and
a solution for cryopreservation of mesenchymal cells or mesenchymal stem cells which substantially comprises a cryoprotectant that contains 3.0 w/v % to 4.5 w/v % of dextran or derivatives thereof or salts thereof, and dimethyl sulfoxide, and a physiological aqueous solution, and does not comprise serum.
2. The cryopreserved product of mesenchymal cells or mesenchymal stem cells according to claim 1 , wherein the mesenchymal cells or mesenchymal stem cells are derived from an umbilical cord or umbilical cord blood.
3. A cryopreservation method of mesenchymal cells or mesenchymal stem cells, comprising:
immersing mesenchymal cells or mesenchymal stem cells in a solution for cryopreservation of mesenchymal cells or mesenchymal stem cells; and
freezing the mesenchymal cells or mesenchymal stem cells while in an immersed state in the solution for cryopreservation,
wherein the solution substantially comprises a cryoprotectant that contains 3.0 w/v % to 4.5 w/v % of dextran or derivatives thereof or salts thereof, and dimethyl sulfoxide, and a physiological aqueous solution, and does not comprise serum.
4. The cryopreserved product of mesenchymal cells or mesenchymal stem cells according to claim 1 , wherein the amount of dimethyl sulfoxide is 1.0 v/v % to 15.0 v/v %.
5. The cryopreserved product of mesenchymal cells or mesenchymal stem cells according to claim 1 , wherein the solution further comprises citric acid or hydrates thereof or salts thereof, and a pH of the solution is 6.0 to 8.0.
6. The cryopreserved product of mesenchymal cells or mesenchymal stem cells according to claim 1 , wherein the solution further comprises glucose.
7. The cryopreserved product of mesenchymal cells or mesenchymal stem cells according to claim 1 , wherein the physiological aqueous solution comprises magnesium, potassium, calcium, sodium, and bicarbonate ions.