Microfluidic system and method of use thereof
This invention concerns an integrated microfluidic system that utilizes microfluidic chip technology to receive a patient sample including cells, expand the cells, reprogram the expanded cells and then store the reprogrammed cells in a microfluidic chip. These microfluidic chips with stored reprogrammed cells may then be used in scenarios of genetic differentiation into specific cell types. Overall this system and workflow is suitable as a hospital based device that will allow the generation of iPSCs from every patient for downstream diagnostic or therapeutic use.
1. A microfluidic system for processing a biological sample comprising
a) one or more microfluidic units to process the sample, the processing comprising:
i) isolating cells from the sample to produce an isolated population of cells;
ii) expanding the isolated population of cells to produce a population of expanded cells;
iii) reprogramming the population of isolated cells or the population of expanded cells; and
iv) storing cells from one or more of (i), (ii) or (iii); and
b) one or more computer memory modules containing instructions, and programmed for controlling one or more of processes (i)-(iv); and
c) one or more computer processor modules programmed to execute the instructions,
d) wherein the one or more microfluidic units comprises a plurality of flow channels.
2. The system of claim 1 , wherein the system is further operable to perform an analysis of the isolated cells of (i), the expanded cells of (ii), the reprogrammed cells of (iii), or a combination thereof.
3. The system of claim 1 , wherein the one or more microfluidic units is further operable to differentiate the iPSC to produce a cell of a desired cell type.
4. The system of claim 3 , wherein the system is further operable to perform an analysis on the cell of the desired cell type.
5. The system of claim 3 , wherein the system is further operable to store the cell of the desired cell type.
6. The system of claim 1 , wherein storing cells comprises freezing the cells.
7. The system of claim 1 , further comprising a graphical user interface.
8. The system of claim 1 , wherein the microfluidic units are wirelessly or electrically coupled.
9. The system of claim 1 , wherein the microfluidic units are fluidly coupled.
10. The system of claim 1 , wherein the microfluidic units are disposed within a unitary housing.
11. The system of claim 1 , wherein the cells are stored on a microfluidic chip.
12. The system of claim 1 , wherein the system further comprises a storage unit for storing the cells at −80° C. or less.
13. The system of claim 1 , wherein the system is further operable to process and store sample data associated with stored cells.
14. The system of claim 1 , wherein cells are stored as master and working cell banks.
15. A method for processing a biological sample comprising: a) applying the sample to the system according to 1; and b) processing the sample with the system, the processing comprising: i) isolating cells from the sample; ii) expanding the isolated cells to produce a population of expanded cells; iii) reprogramming the isolated or expanded cells; and iv) storing cells from any of (i), (ii) or (iii), thereby processing the biological sample.
16. The method of claim 15 , further comprising differentiating the reprogrammed cells to produce a cell of a desired cell type.
17. The method of claim 16 , further comprising analyzing the isolated cells of (i), the expanded cells of (ii), the reprogrammed cells of (iii), the cell of a desired cell type, or a combination thereof.
18. A method of treating a disease or disorder in a subject comprising: a) obtaining a sample from the subject; b) applying the sample to the system according to claim 1 ; c) processing the sample with the system, the processing comprising: i) isolating cells from the sample; ii) expanding the isolated cells to produce a population of expanded cells; iii) reprogramming the isolated or expanded cells; iv) differentiating the reprogrammed cells to a desired cell type; and v) storing cells from any of (i), (ii), (iii) or (iv); and d) administering the subject a cell of any of (i)-(v), thereby treating the disease or disorder in the subject.
19. The method of claim 18 , further comprising analyzing the isolated cells of (i), the expanded cells of (ii), the reprogrammed cells of (iii), the cell of a desired cell type, or a combination thereof.