H5 avian influenza vaccine strain which differentiates infected from vaccinated animals, preparation method therefor, and application
Provided are an H5 avian influenza vaccine strain which differentiates infected from vaccinated animals, a preparation method therefor, and an application. The vaccine strain uses an NA protein of influenza B as a label, and has application value and public health significance for the prevention, control and decontamination of H5 avian influenza.
1. An H5 avian influenza vaccine strain, comprising a label gene sequence and an H5 subtype HA gene or a mutated H5 subtype HA gene, wherein the label gene sequence comprising a DNA sequence coding an influenza B virus NA protein extracellular region amino acid sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 6, or comprising a DNA sequence coding an amino acid sequence having at least 90% homology, at least 92% homology, at least 95% homology, or at least 98% homology with the extracellular region amino acid sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 6;
alternatively, the label gene sequence comprising a DNA sequence set forth in SEQ ID NO: 2 or SEQ ID NO: 7 coding the extracellular region amino acid sequence in influenza B virus NA gene, or comprising a sequence having at least 90% homology, at least 92% homology, at least 95% homology, or at least 98% homology with the DNA sequence set forth in SEQ ID NO: 2 or SEQ ID NO: 7;
and wherein the mutated H5 subtype HA gene has been mutated to encode RETRGLF (SEQ ID NO: 9) in place of RERRRKRGLF (SEQ ID NO: 8).
2. The vaccine strain of claim 1 , wherein the influenza B virus comprises influenza B viruses of Victoria group and Yamagata group.
3. The vaccine strain of claim 2 , wherein the influenza B virus comprises virus strains B/Massachusetts/2/2012, B/Brisbane/60/2008, B/Yamagata/16/1988, or B/Malaysia/2506/04.
4. The vaccine strain of claim 1 , wherein the label gene sequence further comprises a packaging signal sequence at both ends, wherein the packaging signal is a packaging signal of H1 subtype NA set forth in SEQ ID NO: 3 or SEQ ID NO: 4, or a packaging signal sequence having at least 80% homology, at least 85% homology, at least 90% homology, or at least 95% homology with the packaging signal of H1 subtype NA.
5. The vaccine strain of claim 1 , wherein the label gene sequence further comprises packaging signal sequences at both ends, wherein the 5′-end packaging signal sequence comprises a noncoding region sequence, an intracellular region sequence, and a transmembrane region sequence.
6. The vaccine strain of claim 5 , wherein the intracellular region sequence encodes 5˜7 amino acids.
7. The vaccine strain of claim 5 , wherein the transmembrane region sequence encodes 24˜32 amino acids.
8. The vaccine strain of claim 5 , wherein the 5′-end packaging signal sequence of the label gene sequence comprises SEQ ID NO:3, or a sequence having at least 80% homology, at least 85% homology, at least 90% homology, or at least 95% homology with SEQ ID NO:3.
9. The vaccine strain of claim 1 , wherein the label gene sequence further comprises packaging signal sequences at both ends, wherein the 3′-end packaging signal sequence comprises SEQ ID NO:4, or a sequence having at least 80% homology, at least 85% homology, at least 90% homology, or at least 95% homology with SEQ ID NO:4.
10. A preparation method of an H5 avian influenza vaccine strain, comprising:
contacting a label gene sequence with an HA gene or a mutated H5 subtype HA gene of H5 avian influenza virus over a reverse genetic system; and
obtaining a recombinant H5 avian influenza vaccine strain;
wherein:
the mutated H5 subtype HA gene has been mutated to encode RETRGLF (SEQ ID NO: 9) In place of RERRRKRGLF (SEQ ID NO: 8);
the label gene sequence comprising a DNA sequence for coding an influenza B virus NA protein extracellular region amino acid sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 6, or comprising a DNA sequence for coding an amino acid sequence comprising at least 90% homology, at least 92% homology, at least 95% homology, or at least 98% homology with the extracellular region amino acid sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 6;
alternatively, the label gene sequence comprising a DNA sequence set forth in SEQ ID NO: 2 or SEQ ID NO: 7 for coding an extracellular region amino acid sequence in influenza B virus NA gene, or comprising a sequence comprising at least 90% homology, at least 92% homology, at least 95% homology, or at least 98% homology with the DNA sequence set forth in SEQ ID NO: 2 or SEQ ID NO: 7.
11. The method of claim 10 , wherein the label gene sequence further comprises packaging signal sequences at both ends.
12. The method of claim 11 , wherein the 5′-end packaging signal sequence of the label gene sequence comprises SEQ ID NO:3, or a sequence having at least 80% homology, at least 85% homology, at least 90% homology, or at least 95% homology with SEQ ID NO:3.
13. The method of claim 11 , wherein the 3′-end packaging signal sequence of the label gene sequence comprises SEQ ID NO:4, or a sequence having at least 80% homology, at least 85% homology, at least 90% homology, or at least 95% homology with SEQ ID NO:4.
14. The method of claim 10 , further comprising 6 additional PR8 internal genes, wherein the 6 additional PR8 internal genes comprise a wild type NS, a mutated ΔNS gene, PB2, PB1, PA, NP, or M; wherein ΔNS is a mutated NS gene, and the nucleotide sequence of ΔNS is as set forth in SEQ ID NO:5.
15. An H5 avian influenza vaccine strain, which is named as H5 avian influenza vaccine candidate strain Re-MuH5-DIVA-ΔNS, has been preserved in China Center for Type Culture Collection, with the preservation number of CCTCC NO: V201741.
16. An avian influenza vaccine, comprising the vaccine strain of claim 15 .