Crystalline forms of (S)-7-(1-(but-2-ynoyl)piperidin-4-yl)-2-(4-phenoxyphenyl)-4,5,6,7-tetrahydropyrazolo[1,5-a]pyrimidine-3-carboxamide, preparation, and uses thereof
The present invention relates to a crystalline form of (S)-7-(1-(but-2-ynoyl)piperidin-4-yl)-2-(4-phenoxyphenyl)-4,5,6,7-tetrahydropyrazolo[1,5-a]pyrimidine-3-carboxamide (Compound 1) for inhibiting Btk, methods of preparation thereof and pharmaceutical compositions, and use of the crystalline form above in the treatment of a disease, or in the manufacturing of a medicament for the treatment of a disease, such as an allergic disease, an autoimmune disease, an inflammatory disease, and a cancer.
1. Crystalline Form A of Compound 1:
wherein the crystalline form is an ethyl acetate solvate; and
wherein the crystalline form is characterized by an X-ray powder diffraction pattern comprising diffraction peaks having angle values (° 2θ) at 18.6°±0.2° 2θ, 19.8°±0.2° 2θ, and 20.6°±0.2° 2θ.
2. The crystalline form of claim 1 , wherein the crystalline form is further characterized by an X-ray powder diffraction pattern comprising additional diffraction peaks having angle values (° 2θ) at 5.3°±0.2° 2θ and 16.90±0.2° 2θ.
3. The crystalline form of claim 1 , wherein the crystalline form is further characterized by an X-ray powder diffraction pattern comprising additional diffraction peaks having angle values (° 2θ) at 5.3°±0.2° 2θ, 10.9°±0.2° 2θ, 16.9°±0.2° 2θ, 21.1°±0.2° 2θ, 22.5°±0.2° 2θ, and 22.8°±0.2° 2θ.
4. The crystalline form of claim 1 , wherein the crystalline form is further characterized by an X-ray powder diffraction pattern comprising additional diffraction peaks having angle values (° 2θ) at 5.3°±0.2° 2θ, 10.9°±0.2° 2θ, 11.2°±0.2° 2θ, 13.4°±0.2° 2θ, 14.4°±0.2° 2θ, 16.9°±0.2° 2θ, 21.1°±0.2° 2θ, 21.7°±0.2° 2θ, 22.5°±0.2° 2θ, 22.8°±0.2° 2θ, 23.6°±0.2° 2θ, and 24.3°±0.2° 2θ.
5. The crystalline form of claim 1 , wherein the crystalline form is further characterized by a differential scanning calorimetry curve in accordance with FIG. 2 B .
6. The crystalline form of claim 1 , wherein the crystalline form is further characterized by a thermogravimetric analysis curve in accordance with FIG. 2 B .
7. A process for preparing crystalline Form A of Compound 1 of claim 1 :
wherein the crystalline form is an ethyl acetate solvate;
wherein the crystalline form is characterized by an X-ray powder diffraction pattern comprising diffraction peaks having angle values (° 2θ) at 18.6°±0.2° 2θ, 19.8°±0.2° 2θ, and 20.6°±0.2° 2θ; and
wherein the process comprises the following steps:
(a) dissolving an amorphous form of Compound 1 in a solvent selected from the group consisting of acetic acid, acetone, acetonitrile, chloroform, dichloromethane, N,N-dimethylacetamide, dimethylsulfoxide, 1,4-dioxane, ethanol, ethyl acetate, isopropanol, isopropyl acetate, methanol, methyl isobutyl ketone, 1-methyl-2-pyrrolidone, tetrahydrofuran, 2-methyltetrahydrofuran, and toluene, or a mixture thereof;
(b) adding an anti-solvent selected from the group consisting of cyclohexane, n-heptane, n-hexane, methyl tert-butyl ether, petroleum ether, and water, or a mixture thereof, to the solution provided in step (a), to induce precipitation; and
(c) maintaining the mixture provided in step (b) at a temperature below 25° C. or at a temperature of 25° C., to precipitate the crystalline Form A of Compound 1 of claim 1 .