Apparatus and method for cell, spore, or virus capture and disruption
Embodiments disclose an apparatus and methods for biological sample processing enabling isolation and enrichment of microbial or pathogenic constituents from the sample. A vessel for sample containment and extraction is further disclosed for engagement with a transducer capable of efficient sample disruption and lysis. Together these components provide a convenient and inexpensive solution for rapid sample preparation compatible with downstream analysis techniques.
1. An apparatus for sample processing comprising:
a transducer that generates sonic or ultrasonic energy of a selected frequency, the transducer further comprising an interface that includes a tapered surface forming a conical cavity; and
a sample collector with a conical portion configured to be received by the cavity and having a sidewall at least partially complementary to the tapered surface, the interface coupling the transducer to the sample collector such that at least a portion of the sonic or ultrasonic energy generated by the transducer can be propagated into an interior compartment of the sample collector through the tapered surface.
2. The apparatus of claim 1 , wherein the sample collector is dimensioned to be removably retained in the cavity with at least a portion of the sample collector sidewall abutting against the interface to propagate the energy generated by the transducer.
3. The apparatus of claim 1 , wherein the sample collector comprises an inlet portion and an outlet portion, the inlet portion dimensioned to receive a sample, the outlet portion dimensioned to dispense at least a portion of the sample.
4. The apparatus of claim 3 , wherein the sample collector further comprises one or more filters to provide selective passage of the sample therethrough.
5. The apparatus of claim 1 , further comprising a sample having biomaterial disposed in the sample collector, wherein the sonic or ultrasonic energy transmitted by the transducer is capable of at least partially lysing the biomaterial to release a quantity of disrupted or lysed material.
6. The apparatus of claim 5 , wherein the biomaterial comprises cells, spores, or viruses from which nucleic acids or proteins are released by disruption or lysis of the sample.
7. The apparatus of claim 5 , wherein the disrupted or lysed material further comprises nucleic acids or proteins.
8. The apparatus of claim 1 , wherein the selected frequency of the sonic or ultrasonic energy generated by the transducer is less than 50 kHz.
9. The apparatus of claim 8 , wherein the selected frequency of the sonic or ultrasonic energy generated by the transducer is less than 20 kHz.
10. The apparatus of claim 1 , wherein the transducer generates heat and cavitation-inducing energy to propagate into the sample collector.
11. The apparatus of claim 1 , further comprising a quantity of sample disposed in the sample collector.
12. The apparatus of claim 11 , wherein the sonic or ultrasonic energy is configured to induce cavitation in the quantity of sample disposed in the sample collector.