Plant promoter for transgene expression
This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a GmTEFs1 gene. Some embodiments relate to a promoter or a 5′ UTR from a GmTEFs1 gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR or a terminator from a GmTEFs1 gene that functions in plants to promote transcription of operably linked nucleotide sequences.
1. A nucleic acid vector comprising a 3′ UTR operably linked to: a) a heterologous polylinker sequence; or b) a non- Glycine max TEFs1 polynucleotide sequence, wherein said 3′ UTR comprises a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:5.
2. The nucleic acid vector of claim 1 , wherein said 3′ UTR is 588 bp in length.
3. The nucleic acid vector of claim 1 , wherein said 3′ UTR consists of a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:5.
4. The nucleic acid vector of claim 1 , further comprising a sequence encoding a selectable maker.
5. The nucleic acid vector of claim 1 , wherein said non- Glycine max TEFs1polynucleotide sequence is a transgene.
6. The nucleic acid vector of claim 5 , wherein the transgene encodes a selectable marker or a gene product conferring insecticidal resistance, herbicide tolerance, expression of an RNAi, nitrogen use efficiency, water use efficiency, or nutritional quality.
7. The nucleic acid vector of claim 1 , further comprising a promoter polynucleotide sequence of SEQ ID NO:2, wherein the promoter sequence is operably linked to said polylinker or said non- Glycine max TEFs1 polynucleotide sequence.
8. The nucleic acid vector of claim 1 , further comprising an intron sequence.
9. The nucleic acid vector of claim 7 , wherein said promoter has constitutive specific expression.