IP Library › Granted Patent US 11,571,401
Granted Patent B2
US 11,571,401 · App. 16/642,178 · Granted Feb 7, 2023

Compounds, compositions, kits, and methods for activating immune cells and/or an immune system response

Inventors: Rukiyah T. Van Dross-Anderson (Winterville, NC); Rene Escobedo (Wilson, NC)
Assignee: East Carolina University
A61K31/164A61P35/00
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Quick Facts
Patent No.
US 11,571,401
App. No.
16/642,178
Granted
Feb 7, 2023
Kind
B2
Abstract

Provided is a method of activating immune cells and/or an immune system response in a subject. The method may comprise administering to a subject an effective amount of a compound of Formula (I): or a prodrug, derivative, and/or salt thereof. In some embodiments, the method comprises contacting an immune cell with a compound of Formula (I) or a prodrug, derivative, and/or salt thereof. Also provided are compounds, compositions, and kits for activating immune cells and/or an immune system response in a subject.

Claims (28)

1. An ex vivo method of activating an immune cell, the ex vivo method comprising contacting the immune cell with a compound of Formula (I):

or a prodrug, derivative, and/or salt thereof to provide an engineered immune cell.

2. The ex vivo method of claim 1 , wherein the prodrug has a structure of Formula (II):

and

wherein contacting the immune cell with the compound of Formula (I) comprises contacting the immune cell with the prodrug having the structure of Formula (II).

3. The ex vivo method of claim 1 , wherein the engineered immune cell is activated to attack and/or kill cancer cells and/or tumor cells.

4. The ex vivo method of claim 1 , wherein the method induces maturation of the immune cell.

5. The ex vivo method of claim 1 , wherein the immune cell is a dendritic cell and the dendritic cell is activated to uptake, process, and/or present pathogen-derived or host-derived antigenic peptides to naive T-cells.

6. The ex vivo method of claim 1 , wherein the immune cell is a T-cell and the method activates the T-cell to produce a multivalent cellular immune response against a given antigen.

7. The ex vivo method of claim 3 , wherein the cancer cells and/or tumor cells are drug resistant cells, metastatic tumor cells, and/or tumor stem cells.

8. An ex vivo method of activating an immune cell, the ex vivo method comprising:

contacting a cancer cell with a compound of Formula (I):

or a prodrug, derivative, and/or salt thereof to provide a drug-treated cancer cell; and

co-culturing the drug-treated cancer cell and an immune cell to provide an activated immune cell.

9. The ex vivo method of claim 1 , wherein the engineered immune cell is not activated to attack and/or kill non-cancer cells and/or non-tumor cells.

10. The ex vivo method of claim 6 , wherein the multivalent cellular immune response is greater than a response achieved with the given antigen alone.

11. The ex vivo method of claim 1 , further comprising administering the engineered immune cell to a subject.

12. The ex vivo method of claim 1 , further comprising culturing the engineered immune cell.

13. The ex vivo method of claim 8 , wherein the prodrug has a structure of Formula (II):

and

wherein contacting the cancer cell with the compound of Formula (I) comprises contacting the cancer cell with the prodrug having the structure of Formula (II).

14. The ex vivo method of claim 8 , wherein the activated immune cell is activated to attack and/or kill cancer cells and/or tumor cells.

15. The ex vivo method of claim 8 , wherein the method induces maturation of the immune cell.

16. The ex vivo method of claim 8 , wherein the immune cell is a dendritic cell and the dendritic cell is activated to uptake, process, and/or present pathogen- derived or host-derived antigenic peptides to naive T-cells.

17. The ex vivo method of claim 8 , wherein the immune cell is a T-cell and the method activates the T-cell to produce a multivalent cellular immune response against a given antigen.

18. The ex vivo method of claim 17 , wherein the multivalent cellular immune response is greater than a response achieved with the given antigen alone.

19. The ex vivo method of claim 14 , wherein the cancer cells and/or tumor cells are drug resistant cells, metastatic tumor cells, and/or tumor stem cells.

20. The ex vivo method of claim 8 , further comprising administering the activated immune cell to a subject.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 2, 2020
From: VAN DROSS-ANDERSON, RUKIYAH T.; ESCOBEDO, RENE
To: EAST CAROLINA UNIVERSITY
Reel/Frame 052300/0019 →
Continuity (2)
Provisional Application 62553510 · Sep 1, 2017
Related Publication 20200306212A1 · Oct 1, 2020