Method of making and using mammalian liver cells for treating hemophilia or lysosomal storage disorder

1. A method of making an isolated mammalian liver cell that expresses a wild-type version of an aberrantly expressed protein that causes hemophilia or a lysosomal storage disease, the method comprising:

(a) introducing a pair of zinc finger nucleases (ZFNs) into the isolated mammalian liver cell such that a target sequence in an endogenous albumin gene is cleaved, and

(b) 7 to 72 hours after step (a), introducing an adeno-associated viral (AAV) vector comprising a donor sequence encoding a wild-type version of the aberrantly expressed protein in a subject with a hemophilia or a lysosomal storage disease into the liver cell such that the donor sequence is integrated into the cleaved target sequence and the liver cell expresses

the wild-type version of the aberrantly expressed protein.

2. The method of claim 1 , wherein the pair of ZFNs is introduced using RNA.

3. The method of claim 2 , wherein the RNA is mRNA.

4. The method of claim 1 , wherein the pair of ZFNs is introduced using one or more AAV vectors.

5. The method of claim 1 , wherein step (b) is performed 7 to 48 hours after step (a).

6. A method for treating hemophilia or a lysosomal storage disease in a mammal, the method comprising:

a) infusing an isolated mammalian liver cell into a mammal that has hemophilia or a lysosomal storage disorder such that treatment of the hemophilia or a lysosomal storage disorder occurs, wherein the mammal aberrantly expresses a protein that causes the hemophilia or a lysosomal storage disorder and the cell has a genome comprising a nucleic acid sequence encoding a wild-type version of the aberrantly expressed protein operably linked to an endogenous albumin promoter.

7. The method of claim 1 , wherein step (b) is performed 7 to 24 hours after step (a).

8. The method of claim 1 , wherein the AAV vector comprises an AAV2 vector, an AAV6 vector or chimeric AAV2/6.