Multi-channel line scanner for fast large field and confocal imaging
A multi-channel line microscope for single molecule Fluorescence In Situ Hybridization (FISH) imaging of a sample. A microscope stage moves a sample across two or more reflected excitation lines positioned relative to each other so that each excitation line excites a spatially distinct horizontal line in the image plane of the sample. A sample is imaged by moving the microscope stage across two or more reflected excitation lines positioned relative to each other so that each excitation line excites a spatially distinct horizontal line in the image plane of the sample. The apparatus and methods of use are suitable for a broad range of applications.
1. A method of fluorescence imaging of single molecules in a sample, comprising:
placing the sample on a microscope stage, wherein the sample comprises one or more targets and one or more probes, wherein the probes are capable of emitting visually detectable signals, and wherein each individual probe is hybridized to a unique target in the sample;
moving the microscope stage in a single direction linearly or circularly;
exciting one or more probes with excitation lines, wherein two or more excitation lines are positioned relative to each other such that each excitation line excites a spatially distinct horizontal line in an image plane of the sample, and wherein two or more excitation lines are positioned about 0.5 μm to about 100 μm apart from each other; and
detecting one or more signals emitted by the one or more probes.
2. The method of claim 1 , wherein moving the microscope stage is at a constant velocity.
3. The method of claim 2 , wherein constant velocity is about 1 pixel per unit of exposure time.
4. The method of claim 1 , wherein the one or more targets are nucleic acids.
5. The method of claim 1 , wherein the one or more targets are proteins or polypeptides.
6. The method of claim 1 , that comprises n rounds of hybridization.
7. The method of claim 6 , wherein the individual probes are capable of emitting at least F types of detectable visual signals, where F≥2 and F n is greater than the number of targets in the sample.
8. The method of claim 1 , wherein a lens focuses light from an excitation source along one direction to form an excitation line, or any geometric shape, or uses a line shaped laser.
9. The method of claim 1 , wherein the one or more signals emitted by the one or more probes are detected by a detector comprising a charge-coupled device (CCD), complementary metal-oxide semiconductor (CMOS) camera or other detector modalities.
10. The method of claim 1 , further comprising analyzing the sample with light sheet microscopy.
11. The method of claim 1 , wherein an air objective is used in detecting one or more signals emitted by the one or more probes.
12. The method of claim 1 , wherein the probes are used in single molecule fluorescence in situ hybridization (FISH) experiments.