Partition processing methods and systems
The disclosure provides methods for separating and/or purifying one or more molecules released from one or more fluid compartments or partitions, such as one or more droplets. Molecules can be released from a fluid compartment(s) and bound to supports that can be isolated via any suitable method, including example methods described herein. The disclosure also provides devices that can aid in isolating supports bound to molecules.
1. A magnetic separation device comprising:
(a) a first magnetic source;
(b) a second magnetic source,
(c) a first receptacle disposed on a first face of the magnetic separation device, wherein the first receptacle is configured to position a vessel relative to the first magnetic source; and
(d) a second receptacle disposed on a second face of the device, wherein the second receptacle is configured to position the vessel relative to a second magnetic source;
wherein the second face of the magnetic separation device is different from the first face of the magnetic separation device, and wherein the second receptacle of the magnetic separation device is different from the first receptacle of the magnetic separation device.
2. The device of claim 1 , wherein the device comprises a plurality of the first receptacles.
3. The device of claim 2 , wherein the device comprises eight, sixteen, or thirty-two of the first receptacles.
4. The device of claim 3 , wherein the device comprises thirty-two of the first receptacles.
5. The device of claim 1 , wherein the device comprises a plurality of the second receptacles.
6. The device of claim 5 , wherein the device comprises eight, sixteen, or thirty-two of the second receptacles.
7. The device of claim 6 , wherein the device comprises thirty-two of the second receptacles.
8. The device of claim 1 , wherein the first magnetic source and the second magnetic source are the same magnetic source.
9. The device of claim 1 , wherein the first magnetic source and the second magnetic source are different.
10. A system comprising the device of claim 1 and the vessel.
11. The system of claim 10 , wherein the vessel is configured to hold a liquid mixture comprising magnetic particles.
12. The system of claim 10 , wherein the vessel is positioned in first receptacle, and the first magnetic source is configured to provide a magnetic field that immobilizes the magnetic particles at a first location within the vessel.
13. The system of claim 10 , wherein the vessel is positioned in the second receptacle, and the second magnetic source is configured to provide a magnetic field that immobilizes the magnetic particles at a second location within the vessel that is different from the first location.
14. The system of claim 10 , wherein the device comprises a plurality of the first receptacles.
15. The system of claim 14 , wherein the device comprises eight, sixteen, or thirty-two of the first receptacles.
16. The system of claim 15 , wherein the device comprises thirty-two of the first receptacles.
17. The system of claim 10 , wherein the device comprises a plurality of the second receptacles.
18. The system of claim 17 , wherein the device comprises eight, sixteen, or thirty-two of the second receptacles.
19. The system of claim 10 , wherein the system comprises thirty-two of the second receptacles.
20. The system of claim 10 , wherein the system comprises a plurality of the vessels.