Adaptive illumination apparatus, method, and applications
A system and method for adaptive illumination, the imaging system comprising an excitation source having a modulator, which generates a pulse intensity pattern having a first wavelength when the excitation source receives a modulation pattern. The modulation pattern is a data sequence of a structural image of a sample. An amplifier of the imaging system is configured to receive and amplify the pulse intensity pattern from the modulator. A frequency shift mechanism of the imaging system shifts the first wavelength of the pulse intensity pattern to a second wavelength. A laser scanning microscope of the imaging system receives the pulse intensity pattern having the second wavelength.
1. An imaging system for adaptive illumination, comprising:
an excitation source having a modulator, which generates a pulse intensity pattern having a first wavelength when the excitation source receives a modulation pattern;
wherein the modulation pattern is a data sequence of a structural image of a sample;
an amplifier configured to receive and amplify the pulse intensity pattern from the modulator;
a frequency shift mechanism which shifts the first wavelength of the pulse intensity pattern to a second wavelength; and
a laser scanning microscope, which receives the pulse intensity pattern having the second wavelength.
2. The imaging system of claim 1 , wherein the frequency shift mechanism a soliton self-frequency shift (SSFS).
3. The imaging system of claim 2 , wherein the SSFS is in a photonic crystal rod.
4. The imaging system of claim 1 , wherein the sample is a neuron.
5. The imaging system of claim 1 , wherein the excitation source is an all-fiber chirped pulse amplifier.
6. The imaging system of claim 1 , wherein the modulator is an electro-optic modulator.
7. A method for adaptive illuminating, comprising the steps of:
generating a high-resolution structural image of a sample;
processing the high-resolution structural image to determine one or more regions of interest;
converting the regions of interest into a data sequence representing a modulation pattern having a first wavelength;
transmitting the modulation pattern to an excitation source;
encoding, at the excitation source, the modulation pattern in time;
amplifying the modulation pattern with an amplifier at the excitation source;
shifting the first wavelength of the modulation pattern to a second wavelength; and
transmitting the modulation pattern having the second wavelength to a laser scanning microscope.
8. The method of claim 7 , further comprising the step of conducting a raster scan of a sample.
9. The method of claim 7 , wherein the amplifier is an Erbium-doped- fiber amplifier.
10. The method of claim 7 , wherein the excitation source is chirped pulse amplifier.
11. The method of claim 7 , wherein the sample is a neuron.
12. The method of claim 7 , wherein the regions of interest are defined by somas of the neuron.