Implantable nerve guidance conduits having polymer fiber guidance channel
View Patent ↗A nerve guidance conduit includes one or more guidance channels formed as porous polymeric structures. The guidance channels are within an outer tubular structure that includes randomly-oriented nanofibers. The guidance channels may have electrospun nanofibers on their inner and outer surfaces in a parallel alignment with the guidance channels. Such aligned nanofibers may also be present on the inner surface of the outer tubular structure. The outer surfaces of the guidance channels and the inner surface of the tubular structure define additional guidance channels. Such a nerve guidance conduit provides augmented surface areas for providing directional guidance and enhancing peripheral nerve regeneration. The structure also has the mechanical and nutrient transport requirements required over long regeneration periods.
1. A method of preparing a nerve guidance conduit, comprising the steps of:
forming a porous sheet of biocompatible material by a solvent casting process;
forming a plurality of substantially parallel surface channels on a surface of the porous sheet by applying parallel rods having micron-scale diameters to the biocompatible material during the solvent casting process;
cutting the porous sheet into a shaped porous sheet having first and second edges opposite each other and substantially parallel to the surface channels;
cutting rectangular pieces away from corners of the shaped porous sheet, the corners being at opposite ends of the first edge, such that the first edge is shorter in length from the second edge;
depositing a first plurality of first nanofibers along the shaped porous sheet and the plurality of surface channels such that the first nanofibers are aligned with the plurality of surface channels and with each other in a substantially parallel arrangement;
winding the shaped porous sheet into a spiral structure such that the first edge is within the spiral structure; and
depositing a second plurality of second nanofibers around the outside of the spiral structure in random orientations, thereby forming a coating of second nanofibers around the spiral structure.
2. The method of claim 1 , wherein the biocompatible material is polycaprolactone.
3. The method of claim 1 , wherein the porous sheet has a plurality of pores.
4. The method of claim 3 , wherein the pores are created in the porous sheet via a salt leaching step.
5. The method of claim 1 , wherein each surface channel of the plurality of surface channels has a width of about 180 um.
6. The method of claim 1 , wherein the first nanofibers are deposited along the shaped porous sheet via electrospinning.
7. The method of claim 1 , wherein the second edge is on the outside of the spiral structure.
8. The method of claim 7 , wherein the second nanofibers are deposited on the outside of the spiral structure via electrospinning.
9. The method of claim 1 , wherein the second nanofibers form a fibrous tube extending about the spiral structure.
10. The method of claim 9 , wherein the fibrous tube has a thickness of about 150 um.
11. The method of claim 1 , wherein the spiral structure has a first end defined by one of the cut away corners of the shaped porous sheet and a second end defined by the other of the cut away corners of the shaped porous sheet.
12. The method of claim 11 , wherein the first end of the spiral structure is located at one end of the nerve guidance conduit and the second end of the spiral structure is located at an opposite end of the nerve guidance structure.
13. The method of claim 12 , wherein the first end of the spiral structure is configured to receive one stump of a severed nerve and the second end of the spiral structure is configured to receive another stump of the severed nerve.
14. The method of claim 11 , wherein the plurality of surface channels extend from the first end of the spiral structure to the second end of the spiral structure.
15. The method of claim 11 , wherein the spiral structure is wound such that it forms an internal guidance channel extending from the first end of the spiral structure to the second end of the spiral structure.
16. The method of claim 1 , wherein the spiral structure is wound such that the surface channels are arranged on an outward oriented wall of the spiral structure.
17. The method of claim 16 , wherein the spiral structure is wound such that it has a plurality of layers.
18. The method of claim 17 , wherein the surface channels are provided on at least two of the layers forming the spiral structure.
19. The method of claim 18 , further comprising the step of depositing a third plurality of nanofibers on inward oriented walls of at least two layers of the spiral structure.
20. The method of claim 16 , further comprising the step of depositing a third plurality of nanofibers on an inward oriented wall of the spiral structure.