System for blood separation by microfluidic acoustic focusing in separation channels with dimensions defined based on properties of standing waves
Systems and methods for cleansing blood are disclosed herein. The methods include acoustically separating undesirable particles bound to capture particles from formed elements of whole blood. After introducing the capture particles to whole blood containing undesirable particles, the whole blood and capture particles are flowed through a microfluidic separation channel. At least one bulk acoustic transducer is attached to the microfluidic separation channel. A standing acoustic wave, imparted on the channel and its contents by the bulk acoustic transducer, drives the formed elements and undesirable particles bound to capture particles to specific aggregation axes. After aggregating the particles, the formed elements exit the separation channel through a first outlet and are returned to the patient. The undesirable particles, bound to the capture particles, exit through a second outlet and can be discarded to saved for later study.
1. A blood cleansing device comprising:
a microfluidic separation channel defined in a thermoplastic and having an upstream end and downstream end, the microfluidic separation channel comprising:
a first inlet configured to introduce flowing whole blood into a proximal end of the microfluidic separation channel, the whole blood including plasma, a plurality of formed elements and a plurality of undesirable particles;
a first outlet at the downstream end of the microfluidic separation channel positioned substantially along a longitudinal axis of the microfluidic separation channel;
a second outlet at the downstream end positioned adjacent a first wall of the microfluidic separation channel; and
an acoustic transducer positioned adjacent to the microfluidic separation channel and configured to operate at a predetermined frequency to generate a standing acoustic wave across a particle migration region of the microfluidic separation channel, wherein a width of the microfluidic separation channel is between about 25% and 35% of an acoustic wavelength in the whole blood at the predetermined frequency.
2. The blood cleansing device of claim 1 , wherein the width of the microfluidic separation channel is between about 30% and about 35% of the acoustic wavelength in the whole blood at the predetermined frequency.
3. The blood cleansing device of claim 1 , wherein a thickness of a wall material of the microfluidic separation channel is between about 35% and about 45% of the acoustic wavelength in the wall material at the predetermined frequency.
4. The blood cleansing device of claim 1 , wherein the standing acoustic wave is generated in a direction substantially transverse to the longitudinal axis of the microfluidic separation channel.
5. The blood cleansing device of claim 1 , further comprising a capture particle injector configured to introduce a plurality of lipid-based capture particles into the whole blood before the whole blood reaches the particle migration region of the microfluidic separation channel.
6. The blood cleansing device of claim 5 , comprising a reservoir in fluidic communication with the capture particle injector.
7. The blood cleansing device of claim 6 , wherein the reservoir contains the plurality of lipid-based capture particles.
8. The blood cleansing device of claim 6 , wherein the reservoir contains a mixture of materials, which when directed by the capture particle injector into the whole blood, form the plurality of lipid-based capture particles.
9. The blood cleansing device of claim 8 , wherein the mixture of materials comprises an affinity molecule, a lipid, and a fluid with a density less than about 1 g/cm3.
10. The blood cleansing device of claim 5 , wherein the plurality of lipid-based capture particles have significantly different acoustophoretic mobility than that of formed elements of blood.
11. The blood cleansing device of claim 5 , wherein the capture particle injector comprises a microfluidic nozzle.
12. The blood cleansing device of claim 5 , wherein the capture particle injector comprises a porous membrane.
13. The blood cleansing device of claim 1 , wherein the second and third outlets merge at a fourth outlet.