Cysteine engineered antigen-binding molecules
A specific antigen-binding member (ABM) comprising a specific antigen-binding moiety and an antibody Fc region comprising a CH2 domain, which is engineered for a cysteine substitution at position 108 and/or 113, wherein numbering is according to the IMGT, and wherein the antibody Fc region does not comprise an antigen-binding CH3 domain; and an ABM conjugate (ABMC) comprising the ABM and at least one heterologous molecule covalently conjugated to one or both of the cysteines at positions 108 and 113 of the CH2 domain.
1. An isolated antigen-binding member conjugate comprising
a specific antigen-binding moiety and an antibody Fc region comprising a human CH2 domain, having an engineered cysteine substitution at position 108 and/or 113 for site-specific drug conjugation, and
(b) at least one heterologous molecule covalently conjugated to one or both of the cysteines at position 108 and 113 of the CH2 domain, wherein numbering is according to the IMGT.
2. The isolated antigen-binding member conjugate of claim 1 , wherein the heterologous molecule is conjugated to one or both of the cysteines at position 108 and 113 of the CH2 domain via a conjugation linker, wherein numbering is according to the IMGT.
3. The isolated antigen-binding member conjugate of claim 2 , wherein the conjugation linker comprises a maleimide group.
4. A preparation comprising the isolated antigen-binding member conjugate of claim 1 .
5. A method of producing the isolated antigen-binding member conjugate an of claim 1 , comprising the steps:
a) providing an antigen-binding member comprising a specific antigen-binding moiety and an antibody Fc region comprising a CH2 domain, having an engineered cysteine substitution at position 108 and/or 113, wherein numbering is according to the IMGT; and
b) reacting at least one thiol group of one or both of the cysteines at position 108 and 113 of the CH2 domain with a heterologous molecule by a site-specific conjugation method.
6. The method of claim 5 , wherein said at least one thiol group is reacting with said heterologous molecule by a Michael reaction, using a conjugation linker comprising a maleimide group.
7. The isolated antigen-binding member conjugate of claim 1 , wherein the heterologous molecule is selected from the group consisting of a drug, a toxin, an enzyme, and a label.
8. The isolated antigen-binding member conjugate of claim 1 , wherein the antigen-binding moiety is fused to the CH2 domain's N-terminus.
9. The isolated antigen-binding member conjugate of claim 1 , wherein the CH2 domain comprises cysteine substitutions, N108C and L113C, wherein numbering is according to the IMGT.
10. The isolated antigen-binding member conjugate of claim 1 , wherein the antigen-binding moiety comprises an antigen-binding portion of an antibody.
11. The isolated antigen-binding member conjugate of claim 1 , wherein the antigen-binding moiety is selected from the group consisting of a Fab, F(ab′)2, scFv, Fd, and Fv.
12. The isolated antigen-binding member conjugate of claim 1 , wherein the antigen-binding moiety is fused to the CH2 domain's N-terminus via a linker and/or hinge region.
13. The isolated antigen-binding member conjugate of claim 1 , wherein of the CH2 domain's C-terminus is fused to a CH3 domain's N-terminus.
14. The isolated antigen-binding member conjugate of claim 1 , wherein the antibody Fc region is of an IgG, IgA, IgM, or IgE isotype.
15. The isolated antigen-binding member conjugate of claim 1 , wherein the antigen-binding member is selected from the group consisting of a monoclonal antibody, a bispecific antibody, and a multispecific antibody.
16. The isolated antigen-binding member conjugate of claim 1 , wherein the antigen-binding moiety specifically recognizes EGFR or HER2.
17. The isolated antigen-binding member conjugate of claim 1 , wherein the antibody Fc region comprises SEQ ID NO: 4, 5, or 6.