Starch aqueous fermentation feedstock and a method for the production thereo
Provided are aqueous fermentation feedstocks comprising glucose monomers at a concentration of less than 50 gram/Liter (g/L) of the total feedstock, water-soluble dextrose oligomers at a concentration in a range between 50 g/L and 300 g/L of the total feedstock; and water. Further provided are methods of production thereof and uses thereof in the production of single cell protein and/or ethanol.
1. A method for producing an aqueous fermentation feedstock comprising glucose monomers at a concentration of less than 50 gram/Liter (g/L) of the total feedstock; water-soluble dextrose oligomers at a concentration in a range between 50 gram/Liter and 300 gram/Liter of the total feedstock; and water, for growth of single cell proteins, the method comprising
(i) providing a starchy grain;
(ii) comminuting said starchy grain to form comminuted starchy grain;
(iii) adding water to said comminuted starchy grain to form an aqueous slurry of said comminuted starchy grain;
(iv)hydrolytically treating said aqueous slurry at a temperature greater than 25 degrees Celsius to form a starchy mash comprising said water soluble dextrose oligomers, said glucose monomers and water-insoluble carbohydrates; and
(v) filtering at least a fraction of said starchy mash through a hydrophobic microfiltration membrane selected from the group consisting of a sintered stainless steel membrane and a polymeric membrane to form an aqueous filtration permeate consisting of said aqueous fermentation feedstock, and a filtration retentate comprising said water-insoluble carbohydrates.
2. The method of claim 1 , wherein the duration of said hydrolytically treating said aqueous slurry is in a range between 30 minutes and 300 minutes.
3. The method of claim 1 , further comprising grinding of said aqueous slurry.
4. The method of claim 1 , further comprising jet-cooking of said aqueous slurry.
5. The method of claim 1 , wherein said hydrolytically treating comprises contacting said aqueous slurry with at least one alpha-amylase enzyme.
6. The method of claim 1 , wherein said microfiltration membrane is sintered stainless steel membrane with ceramic coating.
7. The method of claim 6 , wherein said ceramic coating comprises titanium oxide.
8. The method of claim 1 , wherein said microfiltration membrane has a pore size in a range between 0.01 micron and 0.5 micron.
9. The method of claim 1 , further comprising washing said retentate with an aqueous washing solution, to form a washed retentate and a wash solution, separating said washed retentate from said wash solution and adding said wash solution to the aqueous fermentation feedstock.
10. The method of claim 9 , wherein said aqueous washing solution comprises water soluble dextrose oligomers.
11. The method of claim 9 , wherein said forming an aqueous slurry comprises blending at least a fraction of said separated wash solution with said comminuted starchy grain.
12. The method of claim 2 , wherein said filtering is carried out according to at least one condition selected from the group consisting of
(i) processing temperature ranging between 37 and 100 degrees Celsius;
(ii) feed pressure ranging between 2 and 10 atmospheres;
(iii) retentate pressure ranging between 2 and 10 atmospheres;
(iv) permeate pressure ranging between 1 and 8 atmospheres;
(v) circulation or feed flow rate that creates a linear velocity within the membranes of at least 5 feet per second; and
(vi)transmembrane pressure drop ranging between 1 and 10 atmospheres or combinations thereof.