Method for disinfecting and tempering grain
Methods for inoculating a grain suitable for use in testing methods of reducing bacterial load are disclosed. Also disclosed are methods of reducing bacterial load on a grain by using elevated temperature and an organic acid. Also disclosed are methods of tempering a grain at an elevated temperature and reduced time as compared to standard tempering methods.
1. A method of reducing bacterial load on grain, the method comprising incubating a mixture including the grain and an organic acid solution for a time and temperature sufficient to provide at least a 2 log reduction in an inoculated grain of the same type, wherein:
the organic acid is lactic acid, peracetic acid, acetic acid, citric acid or salicylic acid;
the time is less than 24 hours; and
the temperature is at least 70° F.
2. The method of claim 1 , wherein the organic acid solution is included in the mixture in an amount sufficient to provide an organic acid content of at least about 2 x 10 −5 moles per gram grain and a moisture content to the mixture of from 12% to 20%.
3. The method of claim 1 , wherein the organic acid solution has a concentration of organic acid of from 1.5% to 15%.
4. The method of claim 1 , wherein the method further comprises a step of preheating the grain prior to incubation with the organic acid solution.
5. The method of claim 1 , wherein the time is at least 1 hour and the temperature is at least 100° F.
6. The method of claim 1 , wherein the time is at least 1 hour and the temperature is from 110° to about 170° F.
7. The method of claim 1 , wherein the organic acid is lactic acid.
8. The method of claim 1 , wherein the method is performed during tempering of the grain.
9. The method of claim 1 , wherein the time is less than 12 hours.
10. The method of claim 1 , wherein the bacterial load includes Shiga toxin producing E. coli (STEC).
11. The method of claim 1 , wherein the temperature is about 70° F.