Anti HLA-G specific antibodies
The present invention relates to antibodies, or antigen-binding fragments thereof, directed against human leukocyte antigen-G (HLA-G) protein and raised against an immunogenic peptide derived from the α3 domain of HLA-G protein. The invention further relates to the immunogenic peptide, and methods for producing said anti-HLA-G specific antibodies.
1. A pharmaceutical composition comprising an anti-human leukocyte antigen G (HLA-G) antibody or an antigen-binding fragment thereof, and a pharmaceutical carrier;
wherein the antibody or antigen-binding fragment comprises:
(a) a heavy chain variable region (VH) comprising a heavy chain complementarity determining region 1 (HC CDR1) of SEQ ID NO: 8, a heavy chain complementarity determining region 2 (HC CDR2) of SEQ ID NO: 10, and a heavy chain complementarity determining region 3 (HC CDR3) of SEQ ID NO: 12; and
(b) a light chain variable region (VL) comprising a light chain complementarity determining region 1 (LC CDR1) of SEQ ID NO: 2, a light chain complementarity determining region 2 (LC CDR2) of sequence KVS and a light chain complementarity determining region 3 (LC CDR3) of SEQ ID NO: 5.
2. The pharmaceutical composition of claim 1 , wherein the VH comprises SEQ ID NO: 64 and the VL comprises SEQ ID NO: 63.
3. The pharmaceutical composition of claim 2 , wherein the antibody is a full-length immunoglobulin G comprising two heavy chains and two light chains.
4. The pharmaceutical composition of claim 1 , wherein the antibody or antigen-binding fragment is humanized or chimeric.
5. The pharmaceutical composition of claim 1 , wherein the VH, the VL, or both the VH and VL comprise human immunoglobulin framework region sequences.
6. The pharmaceutical composition of claim 1 , wherein the antibody comprises an immunoglobulin constant region.
7. The pharmaceutical composition of claim 1 , wherein the antigen-binding fragment is a Fv, a dsFv, a scFv, a Fab, a Fab′, or a F(ab′) 2 .
8. The pharmaceutical composition of claim 1 , wherein the antibody is monoclonal.
9. The pharmaceutical composition of claim 1 , wherein the antibody or antigen-binding fragment is linked to a cytotoxic agent.