Methods and systems for analyzing nucleic acid molecules
Processes and materials to detect cancer from a biopsy are described. In some cases, cell-free nucleic acids can be sequenced, and the sequencing result can be utilized to detect sequences derived from a neoplasm. Detection of somatic variants occurring in phase can indicate the presence of cancer in a diagnostic scan and a clinical intervention can be performed.
1. A method comprising:
(a) obtaining a biological sample from a subject;
(b) selectively enriching the biological sample for at least 1,000 cell-free DNA molecules from genomic regions known to harbor phased variants;
(c) obtaining, by a computer system, sequencing data for the at least 1,000 cell-free DNA molecules from a subject; and
(d) processing, by the computer system, the sequencing data to identify one or more cell-free DNA molecules of the at least 1,000 cell-free DNA molecules, wherein identifying the one or more cell-free DNA molecules comprises aligning reads corresponding to each of the at least 1,000 cell-free DNA molecules to a reference human genome, wherein each of the one or more cell-free DNA molecules comprises a plurality of phased variants relative to a sequence from the reference genome, wherein at least 10% of the one or more cell-free DNA molecules comprises a first phased variant of the plurality of phased variants and a second phased variant of the plurality of phased variants that are separated by at least one nucleotide.
2. The method of claim 1 , further comprising separating, in silico, (i) at least a portion of the identified one or more cell-free DNA molecules from (ii) one or more other cell-free DNA molecules of the plurality of cell-free DNA molecules that are not identified to comprise the plurality of phased variants.
3. The method of claim 1 , wherein the method does not comprise barcode-mediated error suppression.
4. The method of claim 1 , wherein at least 50% of the one or more cell-free DNA molecules comprise a first phased variant and a second phased variant that are separated by at least one nucleotide.
5. The method of claim 4 , wherein 100% of the one or more cell-free DNA molecules comprise a first phased variant and a second phased variant that are separated by at least one nucleotide.
6. The method of claim 1 , wherein the first and second phased variants are separated by at least 2 nucleotides.
7. The method of claim 1 , wherein the first phased variant and the second phased variant are separated by at most 160 nucleotides.
8. The method of claim 1 , further comprising sequencing the at least 1,000 cell-free DNA molecules from the subject.
9. The method of claim 1 , wherein selectively enriching the biological sample for at least 1,000 cell-free DNA molecules from genomic regions known to harbor phased variants in a population of individuals or in a given individual comprises enriching from genomic regions that had been known to experience aberrant somatic hypermutation in a B-cell lymphoma.
10. The method of claim 9 , wherein the aberrant somatic hypermutation is driven by activation-induced deamination.
11. The method of claim 9 , wherein the genomic regions are genomic regions that had been identified as regions susceptible to APOBEC-mediated aberrant somatic hypermutation.
12. The method of claim 1 , wherein the genomic regions are genomic regions known to harbor phased variants based on an evaluation of a population of individuals.
13. The method of claim 1 , wherein the genomic regions are genomic regions known to harbor phased variants in a previously obtained sample from the subject.
14. The method of claim 13 , wherein the previously obtained sample is a tumor DNA-containing sample.