Composition for cell transplant, and method for cell transplant
Provided are a composition for cell transplant and a method for cell transplant, both of which enable a myocardial tissue to favorably retain cardiac myocytes and/or cardiac progenitors and can improve the persistence and proliferation of transplanted cells. The composition for cell transplant of the present invention is a composition for cell transplant, containing cells and an aqueous solution containing a protein (A), the cells including a cardiac myocyte and/or a cardiac progenitor, the protein (A) having a degree of hydrophobicity of 0.2 to 1.2, the protein (A) containing a polypeptide chain (Y) and/or a polypeptide chain (Y′), the protein (A) containing 1 to 100 polypeptide chains as a total of the polypeptide chain (Y) and the polypeptide chain (Y′), the polypeptide chain (Y) being a polypeptide chain having 2 to 100 continuous amino acid sequences (X), the amino acid sequence (X) having any one of a VPGVG sequence (1) corresponding to an amino acid sequence of SEQ ID NO: 1, a GVGVP sequence (2) corresponding to an amino acid sequence of SEQ ID NO: 2, a GPP sequence, a GAP sequence, and a GAHGPAGPK sequence (3) corresponding to an amino acid sequence of SEQ ID NO: 3, the polypeptide chain (Y′) being a polypeptide chain having a structure in which 0.1 to 5% amino acid residues in the polypeptide chain (Y) are replaced by a lysine residue and/or an arginine residue and including 1 to 100 residues as a total of the lysine residue and the arginine residue.
1. A composition for cell transplant, comprising cells and an aqueous solution containing a protein (A),
the cells including a cardiac myocyte and/or a cardiac progenitor,
the protein (A) having a degree of hydrophobicity of 0.2 to 1.2,
the protein (A) containing a polypeptide chain (Y) and/or a polypeptide chain (Y′),
the protein (A) containing 1 to 100 polypeptide chains as a total of the polypeptide chain (Y) and the polypeptide chain (Y′),
the polypeptide chain (Y) being a polypeptide chain having 2 to 100 continuous amino acid sequences (X), the amino acid sequence (X) having any one of a VPGVG (SEQ ID NO: 1), a GVGVP (SEQ ID NO: 2), a GPP sequence, a GAP sequence, and a GAHGPAGPK (SEQ ID NO: 3),
the polypeptide chain (Y′) being a polypeptide chain having a structure in which 0.1 to 5% amino acid residues in the polypeptide chain (Y) are replaced by a lysine residue and/or an arginine residue and including 1 to 100 residues as a total of the lysine residue and the arginine residue,
wherein the protein (A) is at least one protein selected from the group consisting of SELP8K protein (SEQ ID NO: 15), SELP0K protein (SEQ ID NO: 16), SLP4.1 protein (SEQ ID NO: 17), ELP 1.1 protein (SEQ ID NO: 18) and a protein having a homology being 80% or more with SELP8K protein, SELP0K protein, SLP4.1 protein or ELP 1.1 protein.
2. The composition for cell transplant according to claim 1 ,
wherein the protein (A) is present at a concentration of 1 to 20 wt % based on a total weight of the composition for cell transplant.
3. The composition for cell transplant according to claim 1 ,
wherein the cells are present at a concentration of 1×10 5 to 1×10 9 pcs/mL based on a total fluid volume of the composition for cell transplant.
4. The composition for cell transplant according to claim 1 ,
wherein the cells are derived from a stem cell.
5. The composition for cell transplant according to claim 1 ,
wherein the cells are derived from a mammal.
6. The composition for cell transplant according to claim 1 ,
wherein the cells are derived from a human pluripotent stem cell.
7. A method for cell transplant, comprising transplanting the composition for cell transplant according to claim 1 in a myocardial tissue of a mammal other than human.
8. A method for cell transplant according to claim 7 ,
wherein 1×10 3 to 1×10 8 cells are transplanted in the myocardial tissue.