Microfluidic devices
In one example in accordance with the present disclosure, a microfluidic device is described. The microfluidic device includes a reservoir to contain a first thermally expandable fluid, a first heater to heat the thermally expandable fluid in the reservoir, a channel extending from the reservoir and connected to the reservoir at a first opening, and a liquid volume obstructing the channel.
1. A microfluidic device comprising:
a first reservoir comprising a thermally expandable fluid;
a second reservoir comprising more thermally expandable fluid;
a heater to selectively heat the thermally expandable fluid in either of the reservoirs;
a channel extending between the two reservoirs; and
a liquid volume to obstruct the channel;
wherein the heater is controllable to oscillate the liquid volume within the channel by alternately heating the thermally expandable fluid in the first and second reservoirs; and
wherein the channel further comprises a first heating zone at a first temperature, and a second heating zone at a second temperature, the first heating zone and the second heating zone being separated by an unheated portion of the channel.
2. The device of claim 1 , wherein the thermally expandable fluid is a gas.
3. The device of claim 1 , wherein the liquid volume comprises a primer.
4. The device of claim 1 , wherein the heater is controllable such that alternating heating of the first reservoir and second reservoir moves the liquid volume between the first heating zone and the second heating zone based on expansion of the thermally expandable fluid in the reservoirs.
5. The device of claim 4 , wherein the channel further comprises a third heating zone and the device comprises an additional liquid volume in the channel separated from the liquid volume.
6. The device of claim 5 , further comprising an immiscible fluid between the first liquid volume and the second liquid volume.
7. The device of claim 1 , further comprising an optical sensor to image a region between two adjacent heating zones.
8. A method comprising:
providing a liquid volume to a microfluidic device, wherein the microfluidic device comprises:
a first reservoir and a second reservoir containing a first thermally expandable fluid and a second thermally expandable fluid, respectively,
one or more heaters to selectively heat the first thermally expandable fluid and the second thermally expandable fluid alternately in the two reservoirs, and
a channel connected and extending between the two reservoirs, wherein the one or more heaters are controllable to oscillate the liquid volume within the channel by alternately heating the first and second thermally expandable fluids in the first and second reservoirs, respectively, and wherein the channel further comprises a first heating zone at a first temperature, and a second heating zone at a second temperature, the first heating zone and the second heating zone being separated by an unheated portion of the channel; and
alternately heating the thermally expandable fluid in the two reservoirs to oscillate a location of the liquid volume in the channel, where a heated thermally expandable fluid expands and pushes the liquid volume in the channel.
9. The method of claim 8 , wherein the location of the liquid volume is adjusted between the first heating zone and the second heating zone in the channel.
10. The method of claim 8 , further comprising detecting an adjustment of the liquid volume in the channel using a sensor.
11. The method of claim 8 , comprising performing a polymerase chain reaction (PCR), wherein:
the liquid volume comprises a primer, an amplification target, and bases, and the one or more heaters of the microfluidic device include a first heater to heat the first thermally expandable fluid in the first reservoir and a second heater to heat the second thermally expandable fluid in the second reservoir; and
heating the first heater and second heater moves the liquid volume back and forth between the first and second heating zones of the channel by expansion of the first thermally expandable fluid and the second thermally expandable fluid in the first reservoir and second reservoir, respectively.
12. The method of claim 11 , further comprising detecting the liquid volume between the first heating zone and the second heating zone using a sensor.
13. The method of claim 12 , wherein the sensor comprises a pair of electrodes.
14. A microfluidic device comprising:
a first reservoir comprising a thermally expandable fluid;
a second reservoir comprising more thermally expandable fluid;
a heater to selectively heat the thermally expandable fluid in either of the reservoirs;
a channel extending between the two reservoirs, wherein the channel comprises multiple heating zones; and
a liquid volume to obstruct the channel;
wherein the heater is controllable to oscillate the liquid volume within the channel by alternately heating the thermally expandable fluid in the first and second reservoirs; and
wherein the heater alternately heats the thermally expandable fluid in the first and second reservoirs to push the liquid volume back and forth between the multiple heated zones to produce a target time-temperature profile.
15. A microfluidic device comprising:
a first reservoir comprising a thermally expandable fluid;
a second reservoir comprising more thermally expandable fluid;
a heater to selectively heat the thermally expandable fluid in either of the reservoirs;
a channel extending between the two reservoirs;
a first liquid volume and a second liquid volume to obstruct the channel; and
an immiscible fluid to separate the first liquid volume and the second liquid volume.
16. The device of claim 15 , wherein the immiscible fluid comprises an immiscible gas.
17. The device of claim 15 , wherein the immiscible fluid comprises an immiscible liquid that allows pressure from the thermally expandable fluid on the first liquid volume to be transferred to the second liquid volume.