Recombinant
Disclosed herein is a process for producing a recombinant Candida cell, which involves genetically engineering a parent Candida cell using a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas)(CRISPR/Cas) system. A recombinant Candida cell obtained using the process and a method for producing D-lactic acid from a biomass using the recombinant Candida cell are also disclosed.
1. A recombinant Candida cell, wherein the recombinant Candida cell is Candida utilis strain D813 having China Center for Type Culture Collection (CCTCC) deposit number M 2019431.
2. A method for producing D-lactic acid from a biomass, comprising:
fermentation of a biomass with the recombinant Candida cell of claim 1 , wherein the biomass comprises a fermentable sugar, and wherein the recombinant Candida cell consumes the fermentable sugar to produce D-lactic acid.
3. The method of claim 2 , wherein the fermentable sugar is selected from the group consisting of glucose, sucrose, fructose, arabinose, galactose, mannose, cellobiose, and combinations thereof.
4. The method of claim 2 , wherein the biomass further comprises a neutralizing agent selected from the group consisting of CaCO 3 , NH 4 OH, NaOH, and combinations thereof.
5. The method of claim 2 , wherein the fermentation is conducted at a temperature ranging from 25° C. to 37° C.
6. The method of claim 2 , wherein the fermentation is conducted at an aeration rate ranging from 0.1 vvm to 1 vvm.