Prussian blue nanoparticles functionalized with immune signals and applications thereof
Biofunctionalized nanocomposites comprised of a core nanoparticle formed of Prussian blue materials, a shell obtained by partially or completely encapsulating the Prussian blue core with a biocompatible coating, and at least one biomolecule attached to, or absorbed to, the biocompatible coating and uses thereof.
1. A biofunctionalized nanocomposite, comprising:
(a) a core comprising Prussian blue materials;
(b) a positively charged biocompatible coating comprising polyethylenimine (PEI) having an average molecular weight (MW) of about 2000 daltons; and
at least one CpG oligodeoxynucleotide attached to the outer surface of the positively charged biocompatible coating.
2. The biofunctionalized nanocomposite of claim 1 , wherein the Prussian blue materials are iron hexacyanoferrate (II) compounds.
3. The biofunctionalized nanocomposite of claim 1 , wherein the Prussian blue materials are represented by general formula (I):
A x B y M 4 [M′(CN) 6 ] z ·n H 2 O (I)
wherein:
A represents at least one of VO 2 , Ca, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Sr, Zr, Nb, Li, Na, K, Rb, Cs, Fr, Tl, Mo, Ru, Rh, Pd, Ag, Cd, In, Lu, Ba, Hf, Ta, W, Os, Pt, Hg, La, Eu, Gd, Tb, Dy and Ho, in any oxidation state and any combination thereof;
B represents at least one of VO 2 , Ca, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Sr, Zr, Nb, Li, Na, K, Rb, Cs, Fr, Tl, Mo, Ru, Rh, Pd, Ag, Cd, In, Lu, Ba, Hf, Ta, W, Os, Pt, Hg, La, Eu, Gd, Tb, Dy and Ho, in any oxidation state and any combination thereof;
M represents at least one of VO 2 , Ca, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Sr, Zr, Nb, Li, Na, K, Rb, Cs, Fr, Tl, Mo, Ru, Rh, Pd, Ag, Cd, In, Lu, Ba, Hf, Ta, W, Os, Pt, Hg, La, Eu, Gd, Tb, Dy and Ho, in any oxidation state and any combination thereof;
M′ represents at least one of VO 2 , Ca, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, Sr, Zr, Nb, Li, Na, K, Rb, Cs, Fr, Tl, Mo, Ru, Rh, Pd, Ag, Cd, In, Lu, Ba, Hf, Ta, W, Os, Pt, Hg, La, Eu, Gd, Tb, Dy and Ho, in any oxidation state and any combination thereof;
x is from 0.1 to about 1;
y is from 0.1 to about 1;
z is from 0.1 to about 4; and
n is from 0.1 to about 24.
4. The biofunctionalized nanocomposite of claim 1 , wherein at least 75% of the outer surface of the positively charged biocompatible coating has a CpG oligodeoxynucleotide attached thereto.
5. The biofunctionalized nanocomposite of claim 1 , wherein the average molecular weight of the PEI is 2000 Daltons.
6. The biofunctionalized nanocomposite of claim 1 , wherein the biofunctionalized nanocomposite is a photothermal therapy agent.
7. The biofunctionalized nanocomposite of claim 6 , wherein the positively charged biocompatible coating further comprises an imaging agent.