Kit and method for measuring measurement target substance in biological sample
An object of the present invention is to provide a kit and a method which are capable of measuring a measurement target substance in a biological sample with high precision in a measurement range from a low concentration range to a high concentration range. According to the present invention, there is provided a kit for measuring a measurement target substance in a biological sample, including a labeled particle having a first binding substance capable of binding to a measurement target substance in a biological sample, and a substrate having a second binding substance capable of binding to any one of the measurement target substance or the first binding substance, in which the labeled particle is a luminescent labeled particle containing at least one kind of compound represented by Formula (1) and a particle. Each symbol in Formula (1) has the meaning described in the present specification.
1. A kit for measuring a measurement target substance in a biological sample, the kit comprising:
a labeled particle having a first binding substance capable of binding to a measurement target substance in a biological sample; and
a substrate having a second binding substance capable of binding to any one of the measurement target substance or the first binding substance,
wherein the labeled particle is a luminescent labeled particle containing at least one compound represented by Formula (2) and a particle,
in Formula (2), Y 1 and Y 2 each represents a fluorine atom; R 3 represents a hydrogen atom; Ar 3 and Ar 4 each independently represents an aryl group, each of which may have a substituent; and
R 4 , R 6 to R 9 , and R 11 each represents a hydrogen atom and each of R 5 and R 10 represents an aryl group which may have a substituent.
2. The kit according to claim 1 ,
wherein the particle is a latex particle.
3. The kit according to claim 1 ,
wherein the labeled particle is a luminescent labeled particle containing at least one energy donor compound represented by Formula (2), at least one energy acceptor compound represented by Formula (2), and a particle.
4. The kit according to claim 3 ,
wherein a molar ratio of the energy donor compound to the energy acceptor compound is 1:10 to 10:1.
5. The kit according to claim 3 ,
wherein a Stokes shift between the donor compound and the acceptor compound is 40 nm or more.
6. The kit according to claim 1 ,
wherein the substrate includes a detection area having the second binding substance.
7. The kit according to claim 6 ,
wherein the detection area is a metal film containing gold.
8. A method for measuring a measurement target substance in a biological sample, the method comprising:
a reaction step of reacting a biological sample with a labeled particle having a first binding substance capable of binding to a measurement target substance;
a capturing step of capturing the labeled particle on a substrate having a second binding substance capable of binding to any one of the measurement target substance or the first binding substance by bringing a reaction product obtained in the reaction step into contact with the substrate; and
a label information acquisition step of acquiring label information related to the measurement target substance,
wherein the labeled particle is a luminescent labeled particle containing at least one compound represented by Formula (2) and a particle,
in Formula (2), Y 1 and Y 2 each represents a fluorine atom; R 3 represents a hydrogen atom; Ar 3 and Ar 4 each independently represents an aryl group, each of which may have a substituent; and
R 4 , R 6 to R 9 , and R 11 each represents a hydrogen atom and each of R 5 and R 10 represents an aryl group which may have a substituent.
9. The method according to claim 8 ,
wherein the particle is a latex particle.
10. The method according to claim 8 ,
wherein the labeled particle is a luminescent labeled particle containing at least one energy donor compound represented by Formula (2), at least one energy acceptor compound represented by Formula (2), and a particle.
11. The method according to claim 10 ,
wherein a molar ratio of the energy donor compound to the energy acceptor compound is 1:10 to 10:1.
12. The method according to claim 10 ,
wherein a Stokes shift between the donor compound and the acceptor compound is 40 nm or more.
13. The method according to claim 8 ,
wherein the substrate includes a detection area having the second binding substance.
14. The method according to claim 13 ,
wherein the detection area is a metal film containing gold.
15. The method according to claim 14 ,
wherein label information related to the measurement target substance is acquired by fluorescence detection due to surface plasmon excitation.