Detergent compositions and uses thereof
The present invention relates to polypeptides having hexosaminidase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A recombinant microbial host cell transformed with multiple copies of a polynucleotide encoding a polypeptide having hexosaminidase activity, wherein
(a) the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the recombinant host cell, and
(b) the polypeptide is selected from the group consisting of:
(i) a polypeptide comprising the amino acid sequence of SEQ ID NO: 7;
(ii) a polypeptide having at least 97% sequence identity to SEQ ID NO: 8;
(iii) a polypeptide having at least 97% sequence identity to SEQ ID NO: 9;
(iv) a polypeptide having at least 80% sequence identity to SEQ ID NO: 10; and
(v) a polypeptide having at least 85% sequence identity to SEQ ID NO: 11.
2. A method of producing a polypeptide having hexosaminidase activity, comprising cultivating the recombinant host cell of claim 1 under conditions conducive for production of the polypeptide.
3. The method of claim 2 , wherein the polypeptide has at least 90% sequence identity to SEQ ID NO: 10.
4. The method of claim 2 , wherein the polypeptide has at least 93% sequence identity to SEQ ID NO: 10.
5. The method of claim 2 , wherein the polypeptide has at least 95% sequence identity to SEQ ID NO: 10.
6. The method of claim 2 , wherein the recombinant host cell is a Gram-positive bacterium selected from the group consisting of Bacillus, Clostridium, Enterococcus, Geobacillus, Lactobacillus, Lactococcus, Oceanobacillus, Staphylococcus, Streptococcus , and Streptomyces.
7. The method of claim 2 , wherein the recombinant host cell is a Bacillus host cell.
8. A recombinant microbial host cell comprising a polynucleotide encoding a polypeptide having hexosaminidase activity, wherein
(a) the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the recombinant host cell, wherein at least one of the one or more control sequences is heterologous to the polynucleotide, and
(b) the polypeptide is selected from the group consisting of:
(i) a polypeptide comprising the amino acid sequence of SEQ ID NO: 7;
(ii) a polypeptide having at least 97% sequence identity to SEQ ID NO: 8;
(iii) a polypeptide having at least 97% sequence identity to SEQ ID NO: 9;
(iv) a polypeptide having at least 80% sequence identity to SEQ ID NO: 10; and
(v) a polypeptide having at least 85% sequence identity to SEQ ID NO: 11.
9. A method of producing a polypeptide having hexosaminidase activity, comprising cultivating the recombinant host cell of claim 8 under conditions conducive for production of the polypeptide.
10. The method of claim 9 , wherein the polypeptide has at least 90% sequence identity to SEQ ID NO: 10.
11. The method of claim 9 , wherein the polypeptide has at least 93% sequence identity to SEQ ID NO: 10.
12. The method of claim 9 , wherein the polypeptide has at least 95% sequence identity to SEQ ID NO: 10.
13. The method of claim 9 , wherein the recombinant host cell is a Gram-positive bacterium selected from the group consisting of Bacillus, Clostridium, Enterococcus, Geobacillus, Lactobacillus, Lactococcus, Oceanobacillus, Staphylococcus, Streptococcus , and Streptomyces.
14. The method of claim 9 , wherein the recombinant host cell is a Bacillus host cell.
15. A recombinant microbial host cell comprising a polynucleotide encoding a polypeptide having hexosaminidase activity, wherein
(a) the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the recombinant host cell, wherein the polynucleotide is heterologous to the recombinant host cell, and
(b) the polypeptide is selected from the group consisting of:
(i) a polypeptide comprising the amino acid sequence of SEQ ID NO: 7;
(ii) a polypeptide having at least 97% sequence identity to SEQ ID NO: 8;
(iii) a polypeptide having at least 97% sequence identity to SEQ ID NO: 9;
(iv) a polypeptide having at least 80% sequence identity to SEQ ID NO: 10; and
(v) a polypeptide having at least 85% sequence identity to SEQ ID NO: 11.
16. A method of producing a polypeptide having hexosaminidase activity, comprising cultivating the recombinant host cell of claim 15 under conditions conducive for production of the polypeptide.
17. The method of claim 16 , wherein the polypeptide has at least 90% sequence identity to SEQ ID NO: 10.
18. The method of claim 16 , wherein the polypeptide has at least 93% sequence identity to SEQ ID NO: 10.
19. The method of claim 16 , wherein the polypeptide has at least 95% sequence identity to SEQ ID NO: 10.
20. The method of claim 16 , wherein the recombinant host cell is a Gram-positive bacterium selected from the group consisting of Bacillus, Clostridium, Enterococcus, Geobacillus, Lactobacillus, Lactococcus, Oceanobacillus, Staphylococcus, Streptococcus , and Streptomyces.
21. The method of claim 16 , wherein the recombinant host cell is a Bacillus host cell.