Non-coding RNAS (NCRNA) for the diagnosis of cognitive disorders
The present invention describes a method for the diagnosis of a cognitive disorder including but not limited to Alzheimer disease in a subject at risk of having or developing a cognitive disorder.
1. A method of detecting levels of expression of lncRNAs in a lncRNA signature, comprising:
(a) isolating a biological sample from a subject, wherein the biological sample is selected from blood, plasma, and serum, and the subject is human;
(b) detecting levels of expression of lncRNAs in a LncRNA signature in the biological sample from said subject, wherein the lncRNA signature comprises 13 lncRNAs of lnc-DLGS5-1:1, lnc-EBLN1-1:4, lnc-FAT1-7:2, lnc-PRR5-5:1, lnc-RBKS-6:1, lnc-FOXD4L5-35:1, lnc-TENM3-3:3, lnc-FAM133B-2:1, lnc-ZNF726-1:3, lnc-AP3M1-1:1, lnc-DUSP10-6:1, lnc-TPPP-1:2, and LINC01206:20.
2. A method for treating Alzheimer's disease, said method comprising:
(i) performing the method of claim 1 ;
(ii) diagnosing the human subject with Alzheimer's disease when the expression levels of each of the lncRNAs in the lncRNA signature are increased or decreased in comparison to reference expression levels of the lncRNAs in control samples obtained from healthy subjects; and
(iii) administering a treatment to the human subject diagnosed with Alzheimer's disease, wherein the treatment is selected from cholinesterase inhibitors, NMDA receptor antagonists, beta-secretase inhibitors, anti-beta-amyloid monoclonal antibodies, modulators of kinases or phosphatases that regulate tau phosphorylation status and anti-tau antibodies.
3. The method of claim 2 , wherein the treatment is selected from rivastigmine, galantamine, donepezil, and memantine.