Pollen polysaccharide extract and application thereof in plant growth
A pollen polysaccharide extract prepared by the following method: mixing rape pollen with water, then heating and stirring for extraction, filtering, mixing filtrates, adding chitosan to a resulting filtrate to obtain a liquid to be clarified, keeping the liquid to be clarified at 60-80° C. for at least 1 h, cooling and allowing to stand, and performing solid-liquid separation to obtain a liquid, namely the pollen polysaccharide extract.
1. A pollen polysaccharide extract for promoting plant growth, wherein the pollen polysaccharide extract is prepared by the following method:
(a) mixing rape pollen with water, and heating and stirring the rape pollen in water at a speed of 3000-4000 r/min at 60-70° C. for 3-5 h,
(b) filtering,
(c) adding chitosan to the filtrate to obtain a liquid to be clarified, wherein the chitosan in the liquid to be clarified has a chitosan concentration of 0.01-0.15 wt %,
(d) keeping the liquid to be clarified at 60-80° C. for at least 1 h,
(e) cooling said liquid and allowing to stand, and
(f) performing solid-liquid separation to obtain a final liquid, which is the pollen polysaccharide extract for promoting plant growth.
2. The pollen polysaccharide extract according to claim 1 , wherein the water is demineralized water.
3. The pollen polysaccharide extract according to claim 1 , wherein the rape pollen and the water are mixed at a weight ratio of 1:1.5-5 in step (a).
4. The pollen polysaccharide extract according to claim 3 , wherein the rape pollen and the water are mixed at a weight ratio of 1:2.
5. The pollen polysaccharide extract according to claim 1 , wherein the chitosan in the liquid to be clarified has a concentration of 0.05-0.1 wt %.
6. The pollen polysaccharide extract according to claim 1 , wherein the chitosan in the liquid to be clarified has a chitosan concentration of 0.08 wt %.
7. The pollen polysaccharide extract according to claim 1 , wherein the liquid to be clarified is kept at 70° C. for 1 h.
8. A method of promoting the growth of roots of a plant, the method comprising:
applying the pollen polysaccharide extract according to claim 1 to the roots of the plant.
9. A method of promoting the growth of roots of a wheat plant, the method comprising:
applying the pollen polysaccharide extract according to claim 1 to the roots of the wheat plant.
10. A method of promoting the growth of plant stems and leaves, the method comprising:
spraying the pollen polysaccharide extract according to claim 1 on the plant leaves, or
applying the pollen polysaccharide extract according to claim 1 to the plant roots.
11. The method according to claim 10 , wherein the plant is a vegetable or a fruit tree.
12. The method according to claim 11 , wherein the plant is baby Chinese cabbage, asparagus lettuce, lettuce, Chinese cabbage, citrus tree, kiwi fruit tree, cherry tree or apple tree.
13. A pollen polysaccharide extract for promoting plant growth, wherein the pollen polysaccharide extract is prepared by the following method:
(a) mixing rape pollen with water, and heating and stirring the rape pollen in water at a speed of 3000-4000 r/min at 60-70° C. for 3-5 h,
(b) filtering,
(c) mixing the filter residue obtained in step (b) with water, heating and stirring the filter residue in water at a speed of 3000-4000 r/min at 60-70° C. for 3-5 h, and filtering,
(d) combining the filtrate of step (b) and the filtrate of step (c) to form a combined filtrate,
(e) adding chitosan to the combined filtrate to obtain a liquid to be clarified, wherein the chitosan in the liquid to be clarified has a chitosan concentration of 0.01-0.15 wt %,
(f) keeping the liquid to be clarified at 60-80° C. for at least 1 h,
(g) cooling said liquid and allowing to stand, and
(h) performing solid-liquid separation to obtain a final liquid, which is the pollen polysaccharide extract for promoting plant growth.