Core-shell microneedle patch for H
Disclosed are compositions and methods for microneedle patches comprising diblock copolymer micelles designed for pH cascade and H 2 O 2 triggered insulin delivery.
1. A microneedle patch comprising diblock copolymer micelles; wherein the diblock copolymer micelles comprise separately insulin and a glucose responsive enzyme; wherein the diblock copolymer comprises mPEG n -poly(2-(dimethylamino)ethyl methacrylate-4-(bromomethyl)phenylboronic acid) m of formula 1 shown below:
(MPEG n -P(DMAEMA-PBA) m ); wherein n can be between 1 and 8,000; and wherein m can be between 1 and 18,000; wherein the microneedles are coated with H 2 O 2 scavenging enzyme; wherein the diblock copolymer micelles further comprise poly(vinyl alcohol) methacrylate (m-PVA); and wherein the insulin dissociates from the micelle in an acidic and oxidative environment.
2. The microneedle patch of claim 1 , wherein the glucose responsive enzyme is glucose oxidase.
3. The microneedle patch of claim 1 , wherein the H 2 O 2 scavenging enzyme comprises catalase.
4. The microneedle patch of claim 1 , wherein the microneedles comprise a core and the diblock copolymer micelles are crosslinked to the microneedle core via non-cleavable covalent bond.
5. A self-regulating insulin delivery system comprising the microneedle patch of claim 1 .
6. A method of treating hyperglycemia in a subject comprising administering to the subject the microneedle patch of claim 1 .
7. The method of claim 6 , wherein the hyperglycemia is a symptom of diabetes.