Plasma based protein profiling for early stage lung cancer diagnosis
The invention provides biomarkers and combinations of biomarkers useful in diagnosing non-small cell lung cancer. Measurements of these biomarkers are inputted into a classification system such as Random Forest to assist in determining the likelihood that an individual has non-small cell lung cancer. Kits comprising agents for detecting the biomarkers and combination of biomarkers, as well as systems that assist in diagnosing non-small cell lung cancer are also provided.
1. A method for assaying a plurality of biomarkers, wherein the method comprises
obtaining a physiological sample from a subject; and
measuring in the physiological sample a set of biomarkers, wherein the set of biomarkers comprises (i) NSE, CXCL9, CEA, CYFRA-21-1, and (ii) at least 9 biomarkers selected from the group consisting of IL-8, MMP-9, sTNFRII, TNFRI, MMP-7, IL-5, Resistin, IL-10, MPO, MCP-1, GRO-Pan, leptin, MIF, sICAM-1, IL-2, SAA, and PDFG-AB/BB, to produce test data comprising a plurality of biomarker measures.
2. The method of claim 1 , wherein each biomarker is a peptide, protein, peptides and proteins bearing post-translational modifications, or a combination thereof.
3. The method of claim 2 , wherein the physiological sample is a biological fluid.
4. The method of claim 3 , wherein the biological fluid is blood, serum, plasma, or a mixture thereof.
5. The method of claim 1 , wherein the biomarker measures are measured by immunoassay.
6. The method of claim 1 , wherein the subject is 45 years old or older, is a long-term smoker, has been diagnosed with indeterminate nodules in the lungs, or a combination thereof.
7. The method of claim 1 , wherein the set of biomarkers contains no more than 50 biomarkers.
8. The method of claim 1 , wherein the set of biomarkers contains no more than 35 biomarkers.
9. A method for assaying a plurality of biomarkers, wherein the method comprises
obtaining a physiological sample from a subject; and
measuring in the physiological sample a set of biomarkers, wherein the set of biomarkers comprises (i) NSE, CXCL9, CEA, CYFRA-21-1, and either sTNFRII or PDFG-AB/BB, and (ii) at least 7 biomarkers selected from the group consisting of IL-8, MMP-9, TNFRI, MMP-7, IL-5, Resistin, IL-10, MPO, MCP-1, GRO-Pan, leptin, MIF, sICAM-1, IL-2, and SAA, to produce test data comprising a plurality of biomarker measures.
10. A method for assaying a plurality of biomarkers, wherein the method comprises
obtaining a physiological sample from a subject; and
measuring in the physiological sample a set of biomarkers, wherein the set of biomarkers comprises (i) NSE, CXCL9, CEA, CYFRA-21-1, sTNFRII, PDFG-AB/BB, and (ii) at least 7 biomarkers selected from the group consisting of IL-8, MMP-9, TNFRI, MMP-7, IL-5, Resistin, IL-10, MPO, MCP-1, GRO-Pan, leptin, MIF, sICAM-1, IL-2, and SAA, to produce test data comprising a plurality of biomarker measures.