Anti-EGFR antibody polypeptide
The present disclosure provides anti-EGFR antibody polypeptides, polynucleotides encoding the same, pharmaceutical compositions comprising the same, and the uses thereof.
1. An antibody polypeptide comprising a heavy chain variable domain that specifically binds to EGFR, wherein the heavy chain variable domain comprises:
a) a heavy chain variable region comprising CDR1 of SEQ ID NO: 1, CDR2 of SEQ ID NO: 2 and CDR3 of SEQ ID NO: 3; or
b) a heavy chain variable region comprising CDR1 of SEQ ID NO: 4, CDR2 of SEQ ID NO: 5 and CDR3 of SEQ ID NO: 6.
2. The antibody polypeptide of claim 1 , wherein the heavy chain variable domain comprises a sequence selected from the group consisting of SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11 and SEQ ID NO: 13.
3. The antibody polypeptide of claim 2 , further comprising an immunoglobulin constant region, optionally a constant region of human Ig, or optionally a constant region of human IgG1.
4. The antibody polypeptide of claim 1 , capable of specifically binding to human EGFR at an EC 50 value of no more than 1 nM as measured by flow cytometry.
5. The antibody polypeptide of claim 1 , capable of specifically binding to human EGFRvIII at an EC 50 value of no more than 2.5 nM as measured by flow cytometry.
6. The antibody polypeptide of claim 1 , capable of specifically binding to Cynomolgus monkey EGFR, and/or mouse EGFR.
7. A pharmaceutical composition comprising the antibody polypeptide of claim 1 , and a pharmaceutically acceptable carrier.