Carbon dioxide bioconversion process
A CO 2 , bioconversion process includes providing a CO 2 containing substrate to a bioreactor, the CO 2 containing substrate including about 5 to about 90 mole % CO 2 ; and fermenting the CO 2 containing substrate with an acetogenic bacteria carrying a sodium translocating ATPase. The medium including less than about 0.01 grams per liter yeast extract, less than about 0.01 grams per liter carbohydrate, a sodium ion concentration provided by a sodium ion feed rate of about 290 to about 8750 μg/gram of cells/minute, and a pH of about 4 to about 6.9.
1. A process comprising:
providing a gaseous substrate to a bioreactor, the gaseous substrate comprising CO 2 and containing about 5 to about 90 mole % CO 2 , and about 0 to about 5 mole % CO;
providing acetogenic bacteria to the bioreactor;
providing sodium ions to the bioreactor through one or more sodium ion sources; and
fermenting the gaseous substrate with the acetogenic bacteria in a fermentation broth comprising the acetogenic bacteria and the one or more sodium ion sources to produce one or more organic acids;
wherein the acetogenic bacteria includes a sodium translocating ATPase that is active during fermentation in the bioreactor,
wherein the fermentation broth includes less than about 0.01 grams per liter yeast extract, and less than about 0.01 grams per liter carbohydrate,
wherein the sodium ions are provided with a sodium feed rate of about 290 to about 8750 μg/gram of cells/minute,
wherein the fermentation broth is maintained at a pH in a range of about 4 to about 6.9,
wherein the process provides a CO 2 conversion of about 75% to about 100%, and
wherein the process provides a specific organic acid productivity of about 10 to about 50 grams organic acid/day/gram of cells.
2. The process of claim 1 , wherein the CO 2 containing gaseous substrate is selected from the group consisting of industrial gases, fermentor gas streams and mixtures thereof.
3. The process of claim 1 , wherein the acetogenic bacteria is selected from the group consisting of Acetobacterium bacteria, Acetogenium kivui, Acetoanaerobium noterae, Acetobacterium woodii, Moorella thermoacetica, Moorella thermoautotrophica, Ruminococcus productus , and combinations thereof.
4. The process of claim 3 , wherein the acetogenic bacteria is Acetobacterium woodii.
5. The process of claim 1 , wherein the sodium ion source is provided by a compound selected from the group consisting of sodium chloride, sodium hydroxide, sodium phosphate, sodium sulfate, sodium nitrate, sodium bicarbonate, sodium bisulfate and mixtures thereof.
6. The process of claim 1 , wherein the organic acid is one or more C1 to C10 organic acid.
7. The process of claim 6 , wherein the organic acid is acetic acid, butyric acid, or mixtures thereof.
8. A process comprising:
providing a gaseous substrate to a bioreactor, the gaseous substrate comprising and CO 2 and H 2 and containing about 5 to about 90 mole % CO 2 , and about 0 to about 5 mole % CO;
providing acetogenic bacteria to the bioreactor;
providing sodium ions to the bioreactor through one or more sodium ion sources; and
fermenting the gaseous substrate with the acetogenic bacteria in a fermentation broth comprising the acetogenic bacteria and the one or more sodium ion sources to produce one or more organic acids;
wherein the acetogenic bacteria includes a sodium translocating ATPase that is active during fermentation in the bioreactor,
wherein the fermentation broth includes less than about 0.01 grams per liter yeast extract, less than about 0.01 grams per liter carbohydrate,
wherein the sodium ions are provided with a sodium feed rate of about 290 to about 8750 μg/gram of cells/minute,
wherein the fermentation broth is maintained at a pH in a range of about 4 to about 6.9,
wherein the process provides a CO 2 conversion of about 75% to about 100%, and
wherein the process provides a specific organic acid productivity of about 10 to about 50 grams organic acid/day/gram of cells.
9. The process of claim 8 , wherein the gaseous substrate is selected from the group consisting of industrial gases, fermentor gas streams and mixtures thereof.
10. The process of claim 8 , wherein the acetogenic bacteria is selected from the group consisting of Acetobacterium bacteria, Acetogenium kivui, Acetoanaerobium noterae, Acetobacterium woodii, Moorella thermoacetica, Moorella thermoautotrophica, Ruminococcus products , and combinations thereof.
11. The process of claim 10 , wherein the acetogenic bacteria is Acetobacterium woodii.
12. The process of claim 8 , wherein the sodium ion source is provided by a compound selected from the group consisting of sodium chloride, sodium hydroxide, sodium phosphate, sodium sulfate, sodium nitrate, sodium bicarbonate, sodium bisulfate and mixtures thereof.
13. The process of claim 8 , wherein the organic acid is one or more C1 to C10 organic acid.
14. The process of claim 13 , wherein the organic acid is acetic acid, butyric acid, or mixtures thereof.