Heterodimeric Relaxin fusions polypeptides
The present invention relates to heterodimeric Relaxin fusion polypeptides, in particular to heterodimeric Relaxin 2 fusion polypeptides and uses thereof. Thus, the invention provides Relaxin fusion polypeptides, nucleic acid molecules, vectors, host cells, pharmaceutical compositions and kits comprising the same and uses of the same including methods of treatment. The polypeptides and compositions of the invention may be useful, in particular, in the treatment of cardiovascular diseases, for example for the treatment of heart failure.
1. A heterodimeric fusion comprising:
(i) a polypeptide according to SEQ ID NO: 11; and
(ii) a polypeptide according to SEQ ID NO: 20,
wherein SEQ ID NO: 11 comprises a first heterodimerisation domain connected to a Relaxin A chain polypeptide;
wherein SEQ ID NO: 20 comprises a second heterodimerisation domain connected to a Relaxin B chain polypeptide;
wherein the first heterodimerisation domain heterodimerises with the second heterodimerisation domain, and wherein the heterodimeric fusion has Relaxin activity.
2. The heterodimeric fusion according to claim 1 , wherein the Relaxin A chain polypeptide and the Relaxin B chain polypeptide are covalently bound by at least one inter-chain disulphide bond.
3. The heterodimeric fusion according to claim 1 , wherein the Relaxin A chain polypeptide and the Relaxin B chain polypeptide are not covalently linked to each other by an amino acid linker.
4. A pharmaceutical composition comprising the heterodimeric fusion of claim 1 and a pharmaceutically acceptable excipient.
5. A kit comprising the pharmaceutical composition of claim 4 .