Interface component for a microfluidic device, assemblies, and method for extracting particles from a sample
All interface component ( 40 ), suitable for cooperating with a microfluidic device ( 1 ), the interface component comprising, one or more elements ( 41 ) which can be selectively connected to a pneumatic system ( 71 a, 71 b) which can provide a positive and/or negative air flow to the one or more elements ( 41 ); wherein each of the one or more elements 141 ) comprises, an input port ( 42 ) which can be selectively fluidly connected to a pneumatic system ( 71 a, 71 b); and a flow restrictor ( 43 ) according to a further aspect of the present invention; the flow restrictor ( 43 ) being arranged in fluid communication with the input port ( 42 ), wherein the flow restrictor ( 43 ) can restrict the flow of fluid through the element ( 41 ); and an aerosol filter ( 49 ) which is arranged to be in fluid communication with the flow restrictor ( 43 ); and wherein the interface component ( 40 ) further comprises one or more outlets ( 45 ), each of the one or more outlets ( 45 ) being in fluid communication with a respective element ( 41 ), so that fluid can flow from the element ( 41 ) out of the interface component ( 40 ) via the one or more outlets ( 45 ); and wherein each of the one or more outlets ( 45 ) can be selectively arranged to be in fluid communication with a respective reservoir ( 105,1 ( 16,107,108 ) of a microfluidic device ( 1 ). There is further provided a corresponding method and assembly for extracting ferromagnetic, paramagnetic and/or diamagnetic particles from a sample.
1. A flow restrictor suitable for use in an interface component, the flow restrictor comprising,
an inlet member which has an inlet channel defined therein;
an outlet member which has an outlet channel defined therein;
wherein the inlet channel and outlet channel are fluidly connected; and
a capillary member which comprises an intermediate channel which is located between the inlet and outlet members, and wherein the intermediate channel is in fluid communication with the inlet channel and outlet channel; and wherein the intermediate channel has dimensions smaller than the dimensions of the inlet and outlet channels;
wherein the flow restrictor comprises a male member and female member which are configured so that they can mechanically cooperate with each other so that the male and female members can be fixed together;
wherein the male member comprises the inlet member, and the female member comprises the outlet member; and
wherein the male and female member each have a pocket which can receive a portion of the capillary member so that a portion of capillary member is contained within the pocket in the male member, and another portion of the capillary member is contained within pocket of the female member.
2. A flow restrictor according to claim 1 wherein at least 0.5 mm of the length of the capillary member extends out of the pocket of the male member.
3. A flow restrictor according to claim 1 further comprising an o-ring located at an interface between the male and female members.
4. A flow restrictor according to claim 3 wherein the capillary member extends through the o-ring.
5. A flow restrictor according to claim 1 wherein said capillary member is composed of glass.
6. A flow restrictor according to claim 1 wherein the depth of the pocket in the male member is such that when the capillary member is positioned into the pocket so that capillary member abuts a base of the pocket, at least 0.5 mm of the length of the capillary member extends out of the pocket of the male member.
7. A flow restrictor according to claim 1 wherein the depth of the respective pockets in the male member and female member is between 1-20 mm.
8. A flow restrictor according to claim 1 wherein the male member further comprises ribbing defined on an outer surface thereof and/or the female member further comprises ribbing defined on an outer surface thereof.
9. A flow restrictor assembly comprising,
a flow restrictor according to claim 1 , wherein the male member and female member can mechanically cooperate such that the pockets in each member align to define a volume which can receive a capillary member; and
a plurality of capillary members each of which has an intermediate channel defined therein; wherein the length of each the capillary members is different such that the lengths of their respective intermediate channels are different; and wherein each of the capillary members being dimensioned such that they can be fully contained within the volume defined by the pockets in the male and female members.